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Monoclonal Antibodies

Anti-TCR Cβ1 [JOVI.1]

Invented by Dr Mike Owen from Independent Pharmaceutical And Biotech Consultant
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Risueño et al. 2008. PLoS One. 3(3):e1747. PMID: 18320063.Figure. Stimulation of living T cells provokes the adoption of a protease-resistant conformation in CD3ε and CD3ζ. (A) CD3 ligation in living cells protects against proteolytic cleavage. Jurkat cells were unstimulated or stimulated for 5 min with 10 µg/ml of either anti-CD3 (OKT3) or an irrelevant antibody (HP2/6, anti-CD4). Detergent lysates were incubated with trypsin as in Fig. 1, and immunoblotting was performed with APA1/1, 448 and M20, an antibody specific for the C-terminal end of CD3ε. (B) Stimulation with certain anti-TCRα/β antibodies promotes the protease-resistant conformation in CD3ε and CD3ζ. Jurkat cells were stimulated for 5 min with the anti-Vβ antibodies BV8 (IgG2b) and C305 (IgM) or with the anti-Cβ antibody Jovi.1 (IgG1). Lysates were incubated with trypsin, and immunoblotting was performed as in A. (C) Differential capacity of the anti-TCRβ antibodies to induce the conformational change determined by the pull-down assay. Jurkat cells were stimulated with an irrelevant anti-CD4 antibody or with the indicated antibodies to the TCR complex, as in B. Lysates were subjected to pull-down with GST-SH3.1 (GST-Nck) or GST. The presence of the TCR in the precipitates was revealed by immunoblotting with anti-CD3ε (M20). TL, total cell lysate.
Adapted from Rossatti et al. 2019. Cells 8(11). PMID: 31690048. Figure. Flow cytometry measurements of calcium flux based on Fluo-3-AM fluorescence in Jurkat T cells stimulated with soluble antibodies (anti-CD3ε, clone OKT3 and anti-CD28 or anti TCRβ, clone JOVI.1) at the indicated concentrations.
Adapted from Chen et al. 2007. J Biol Chem; 282(48):35361-72. PMID: 17897956. Figure. Jurkat T cells pretreated as indicated were lysed, immunoprecipitated (IP) for Nck, and Western blotted (WB) as indicated.
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Catalogue Number 151289
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets TCR Cβ1
Reactivity Human
Relevance Monoclonal antibody which aids investigation into T-cell related diseases.

Background and Research Application
The T cell receptor is expressed on all mature T cells. The T cell receptor is heterodimer of alpha/beta or gamma/delta chains, expressed on all mature T cells. TCR’s recognize antigen peptides bound to MHC molecules, providing the basis of antigen specific response by T cells. Genetic mutations involving the T cell receptor β locus have been associated with T cell lymphomas.

JOVI.1 can be used for studies of T-cell mediated diseases, including autoimmunity and allergy. Antibody JOVI·1 recognised a determinant on the majority of TCRs, staining 50-75% of peripheral blood T cells and T cell lines expressing different Vβ regions.
Host Mouse
Immunogen Human HA1.7 TCR beta chain expressed on transgenic mouse cells.
Immunogen UniProt ID P01850
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
JOVI.1 monoclonal antibody that targets TCRBC1+ cells in a selective manner by recognizing 2 amino acid residues specifically exposed in TCRBC1 but not in TCRBC2.

Concentration
1mg/ml as standard
Research Area Cell Type or Organelle Marker, Immunology

References

There are 10 reference entries for this reagent.

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References: 10 entries

Rossatti et al. 2019. Cells. 8(11):. PMID: 31690048.

FACS

View  

Beck et al. 2018. Hum Vaccin Immunother. 14(12):2864-2873. PMID: 30111232.

View  

Tesio et al. 2018. Hemasphere. 2(2):e38. PMID: 31723766.

View  

T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation.

Europe PMC ID: 18320063

View  

Risueño et al. 2008. PLoS One. 3(3):e1747. PMID: 18320063.

View  

Chen et al. 2007. J Biol Chem. 282(48):35361-72. PMID: 17897956.

WB

View  

Gil et al. 2002. Cell. 109(7):901-12. PMID: 12110186.

IP

View  

Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.

Europe PMC ID: 12110186

View  

Viney et al. 1992. Hybridoma. 11(6):701-13. PMID: 1284120.

View  

Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice.

Europe PMC ID: 1284120

View  

Add a reference

References: 10 entries

Rossatti et al. 2019. Cells. 8(11):. PMID: 31690048.

FACS

View  

Beck et al. 2018. Hum Vaccin Immunother. 14(12):2864-2873. PMID: 30111232.

View  

Tesio et al. 2018. Hemasphere. 2(2):e38. PMID: 31723766.

View  

T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation.

View  

Risueño et al. 2008. PLoS One. 3(3):e1747. PMID: 18320063.

View  

Chen et al. 2007. J Biol Chem. 282(48):35361-72. PMID: 17897956.

WB

View  

Gil et al. 2002. Cell. 109(7):901-12. PMID: 12110186.

IP

View  

Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.

View  

Viney et al. 1992. Hybridoma. 11(6):701-13. PMID: 1284120.

View  

Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice.

View  

Add a reference

Inventor Information