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Anti-p53 [Pab DO-1]

Invented by David Lane at Karolinska Institutet

Info

Catalogue Number 151147
Applications ChIP IHC IF IP WB
Antigen/Gene or Protein Targets p53 (N terminus)
Synonyms P53, Trp53, pantropic
Reactivity Human
Relevance p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is involved in many mechanisms of anticancer function, and plays a role in apoptosis, genomic stability, and inhibition of angiogenesis. Understanding the functions of mutant p53 can help in the development of new therapeutic approaches that may be useful in a broad range of cancer types.
Host Mouse
Immunogen Recombinant human wild type p53 protein expressed in E.coli
Subclass IgG2a
Positive Control MDA-MB-231 cell line
Molecular Weight (kDa) 53
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Epitope maps between amino acid residues 11-25 of p53 of human origin
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling

References: 18 entries

Rizzo et al. 2016. Nucleic Acids Res. :. PMID: 27923994.

WB

SIRT6 interacts with TRF2 and promotes its degradation in response to DNA damage.

Europe PMC ID: 27923994

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Lysines in the tetramerization domain of p53 selectively modulate G1 arrest.

Europe PMC ID: 27210019

İlhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.

Europe PMC ID: 27016721

Targeting polo-like kinase 1 suppresses essential functions of alloreactive T cells.

Europe PMC ID: 26724940

Berges et al. 2016. Immunol Res. :. PMID: 26724940.

Kloet et al. 2015. Biochem J. 469(2):289-98. PMID: 25990325.

FOXO target gene CTDSP2 regulates cell cycle progression through Ras and p21(Cip1/Waf1).

Europe PMC ID: 25990325

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP WB

Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters.

Europe PMC ID: 24928858

Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.

IF

Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.

Europe PMC ID: 11331871

Stephen et al. 1995. J Mol Biol. 248(1):58-78. PMID: 7537340.

Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions.

Europe PMC ID: 7537340

Vojtĕsek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.

An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.

Europe PMC ID: 1378473


Add a reference

References: 18 entries

Rizzo et al. 2016. Nucleic Acids Res. :. PMID: 27923994.

WB

SIRT6 interacts with TRF2 and promotes its degradation in response to DNA damage.

Europe PMC ID: 27923994

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Lysines in the tetramerization domain of p53 selectively modulate G1 arrest.

Europe PMC ID: 27210019

İlhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.

Europe PMC ID: 27016721

Targeting polo-like kinase 1 suppresses essential functions of alloreactive T cells.

Europe PMC ID: 26724940

Berges et al. 2016. Immunol Res. :. PMID: 26724940.

Kloet et al. 2015. Biochem J. 469(2):289-98. PMID: 25990325.

FOXO target gene CTDSP2 regulates cell cycle progression through Ras and p21(Cip1/Waf1).

Europe PMC ID: 25990325

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP WB

Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters.

Europe PMC ID: 24928858

Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.

IF

Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.

Europe PMC ID: 11331871

Stephen et al. 1995. J Mol Biol. 248(1):58-78. PMID: 7537340.

Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions.

Europe PMC ID: 7537340

Vojtĕsek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.

An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.

Europe PMC ID: 1378473


Add a reference

References: 18 entries

Rizzo et al. 2016. Nucleic Acids Res. :. PMID: 27923994.

WB

SIRT6 interacts with TRF2 and promotes its degradation in response to DNA damage.

Beckerman et al. 2016. Cell Cycle. :1-14. PMID: 27210019.

IF

Lysines in the tetramerization domain of p53 selectively modulate G1 arrest.

İlhan et al. 2016. J Vet Diagn Invest. :. PMID: 27016721.

Expression of p53 protein, Jaagsiekte sheep retrovirus matrix protein, and surfactant protein in the lungs of sheep with pulmonary adenomatosis.

Targeting polo-like kinase 1 suppresses essential functions of alloreactive T cells.

Berges et al. 2016. Immunol Res. :. PMID: 26724940.

Kloet et al. 2015. Biochem J. 469(2):289-98. PMID: 25990325.

FOXO target gene CTDSP2 regulates cell cycle progression through Ras and p21(Cip1/Waf1).

Loughery et al. 2014. Nucleic Acids Res. 42(12):7666-80. PMID: 24928858.

ChIP WB

Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters.

Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.

IF

Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.

Stephen et al. 1995. J Mol Biol. 248(1):58-78. PMID: 7537340.

Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions.

Vojtĕsek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.

An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53.


Add a reference


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