Cancer Research Technology
Log in Register
Menu

Info

Catalogue Number 153402
Applications IHC IP WB
Antigen/Gene or Protein Targets Human TP53 / p53
Synonyms p53
Reactivity Human
Relevance Mouse anti human p53 antibody, clone DO-11 recognizes the human cellular tumor antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637). DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnock et al. 2011) and has been used successfully for detection of p53 by western blotting (Wei et al. 2012).
Host Mouse
Immunogen Recombinant human p53
Positive Control Colon or breast carcinoma tissue
Subclass IgG1
Molecular Weight (kDa) 53
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Immunology

References

There are 8 reference entries for this reagent.

View All References

References: 8 entries

Wei et al. 2012. Proc Natl Acad Sci U S A. 109(38):E2543-50. PMID: 22927405.

Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses.

Europe PMC ID: 22927405

Warnock et al. 2011. Cancer Biol Ther. 12(12):1059-68. PMID: 22157150.

Aurora A mediates cross-talk between N- and C-terminal post-translational modifications of p53.

Europe PMC ID: 22157150

Coomber et al. 2001. Clin Cancer Res. 7(9):2802-8. PMID: 11555596.

Isolation of human antibodies against the central DNA binding domain of p53 from an individual with colorectal cancer using antibody phage display.

Europe PMC ID: 11555596

Palecek et al. 2001. Eur J Biochem. 268(3):573-81. PMID: 11168396.

Binding of p53 and its core domain to supercoiled DNA.

Europe PMC ID: 11168396


Add a reference

References: 8 entries

Wei et al. 2012. Proc Natl Acad Sci U S A. 109(38):E2543-50. PMID: 22927405.

Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses.

Warnock et al. 2011. Cancer Biol Ther. 12(12):1059-68. PMID: 22157150.

Aurora A mediates cross-talk between N- and C-terminal post-translational modifications of p53.

Coomber et al. 2001. Clin Cancer Res. 7(9):2802-8. PMID: 11555596.

Isolation of human antibodies against the central DNA binding domain of p53 from an individual with colorectal cancer using antibody phage display.

Palecek et al. 2001. Eur J Biochem. 268(3):573-81. PMID: 11168396.

Binding of p53 and its core domain to supercoiled DNA.


Add a reference