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Recombinant CHO-K1 cell line with integrated Technology for enhanced production of Human Erythropoietin protein, in Adherent Cells

Invented by Saiyed Taslimarif , Patel Paresh Brijalal , Mukhopadhyaya Rabindranath
Invented at The Centre for Cellular and Molecular Platforms C-CAMP

Info

Catalogue Number 160884
Antigen/Gene or Protein Targets Erythropoietin (EPO)
Parental Line CHO-K1
Synonyms haematopoietin, or haemopoietin
Host Hamster
Tissue Ovary
Disease Keywords Therapeutics, Haematopoiesis, Anemia
Production Details The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant.
Conditional No
Research Area Drug Discovery & Development
Recommended Growing Conditions F12 media supplemented with 10% FBS and 1% of 5000U/ml Penstrep (penicillin G and streptomycin) antibiotic. The cells are grown in a CO2 incubator at 37°C with 5% CO2.

References

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References: 1 entry

A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)


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References: 1 entry

A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)


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Inventor Information

Inventors

Saiyed Taslimarif

Saiyed Taslimarif

Patel Paresh Brijalal

Patel Paresh Brijalal

Mukhopadhyaya Rabindranath

Mukhopadhyaya Rabindranath

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