Cat. #160884
Recombinant CHO-K1 cell line with integrated Technology for enhanced production of Human Erythropoietin protein, in Adherent Cells
Cat. #: 160884
Organism: Hamster
Tissue: Ovary
Disease: Therapeutics
£575.00
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Contributor
Inventor: Saiyed Taslimarif ; Patel Paresh Brijalal ; Mukhopadhyaya Rabindranath
Institute: The Centre for Cellular and Molecular Platforms C-CAMP
Tool Details
*FOR RESEARCH USE ONLY
- Name: Recombinant CHO-K1 cell line with integrated Technology for enhanced production of Human Erythropoietin protein, in Adherent Cells
- Alternate name: haematopoietin, or haemopoietin
- Parental cell: CHO-K1
- Organism: Hamster
- Tissue: Ovary
- Donor: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant.
- Disease: Therapeutics
- Conditional: No
- Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant.
Target Details
- Target: Erythropoietin (EPO)
Handling
- Growth medium: F12 media supplemented with 10% FBS and 1% of 5000U/ml Penstrep (penicillin G and streptomycin) antibiotic. The cells are grown in a CO2 incubator at 37?°C with 5% CO2.
- Temperature: 37° C
- Atmosphere: 5% CO2
References
- A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)
