| Catalogue Number | 160871 |
| Antigen/Gene or Protein Targets | monoglyceride lipase; monoacyl glycerol lipase |
| Synonyms | MGL, Mgll, Magl |
| Model | Knock-Out |
| Relevance | Useful model to study MAGL-deficiency. Animals might be useful for studies, e.g. on energy metabolism or endocannabinoid signalling. |
| Production Details | A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed Mgll exon 3 and 4 and the selection cassette. Further details are available upon request |
| Breeding Information | Good breeder. Homozygous mice may be bred together to maintain a live colony. |
| Conditional | No |
| Growth/Phenotype Keywords | These mice display a mildly attenuated peripheral lipolysis, and are resistant to diet-induced insulin resistance and hepatic steatosis. Further, these mice display a desensitization of intestinal cannabinoid receptor type 1, which leads to accelerated colonic propulsion. |
| Strain | C57BL/6J |
| Mouse Genetic Background/Cross History | These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are backcrossed regularly to C57BL/6J to avoid generation of subpopulations. |
| Zygosity | Homozygous |
| Research Area | Metabolism, Cell Signaling & Signal Transduction |
Invented by Robert Zimmermann at University of Graz
|
Robert Zimmermann |
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|
Franz Radner |
