MAGL-Flox mouse

Datasheet

References (2)

Inventor Info

Info

Catalogue Number	160870
Antigen/Gene or Protein Targets	monoglyceride lipase; monoacyl glycerol lipase
Synonyms	MGL, Mgll, Magl
Model	Conditional KO
Relevance	Useful model for the generation of tissue/cell specific MAGL-deficient mice. Animals might be useful for studies, e.g. on energy metabolism or endocannabinoid signalling.
Production Details	A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed the selection cassette leaving Mgll exon 3 and 4 floxed. Further details are available upon request.
Breeding Information	Good breeder. homozygous mice may be bred together to maintain a live colony.
Conditional	Yes
Conditional Description	Targeted, conditional ready
Growth/Phenotype Keywords	These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
Strain	C57BL/6J
Mouse Genetic Background/Cross History	These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
Zygosity	Homozygous
Research Area	Metabolism, Cell Signaling & Signal Transduction

There are 2 reference entries for this reagent.

View All References

21454566

26565024

Add a reference

21454566

26565024

Add a reference

Robert Zimmermann

Franz Radner

Add an inventor

	University of Graz
	11 reagents listed on Ximbio

This reagent can be obtained from the following supplier. Click through to their website for more details.