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4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) biomolecular fluorophore small molecule (tool compound)
RECENTLY UPDATED

Invented by Susan Bane , Kamalika Mukherjee
Invented at Binghamton University

Info

Catalogue Number 160832
Synonyms TFCH
Type Fluorescent Probe
Relevance 4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) is a novel fluorescent biocompatible probe has been developed to detect oxidative damage in live cells and tissues in as little as 5 minutes. TO accomplish this, TFCH utilizes the irreversible reaction of radicals with biomolecules that result in the covalent addition of carbonyls to biomolecules. This probe has been shown to outperform other commercially available toxicity and reactive oxygen species assays.

This probe has been shown to be highly sensitive. The limit of detection is orders of magnitude smaller than other previously reported fluorophores resulting in less reagent needed to detect carbonylation.

TFCH is rapid, live cell and tissue compatible and also be used in fixed cells and tissues.
Key Attributes Detection of Oxidative Stress damage in a myriad of cells and tissues.

Detection of an early biomarker for disease, toxicity, cell and tissue damage.

Use in live cells and tissues, fixed cells and tissues and existing biopsy material.
In vivo applications Renal tissue imaging
In vitro applications Live cell screening
Research Area Drug Discovery & Development, Fluorescent Cell Imaging

References

There are 2 reference entries for this reagent.

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References: 2 entries

A Highly Sensitive Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue

Mukherjee et al. 2021. ACS Sens. 6(7):2523-2528. PMID: 34214393.


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References: 2 entries

A Highly Sensitive Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue

Mukherjee et al. 2021. ACS Sens. 6(7):2523-2528. PMID: 34214393.


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Inventor Information

Inventors

Susan Bane

Susan Bane

Kamalika Mukherjee

Kamalika Mukherjee

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