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Tnao38 subclone cell line

Invented at Boyce Thompson Institute

Info

Catalogue Number 160776
Parental Line High Five (T. ni)
Synonyms BTI-Tnao38
Relevance The insect cell based baculovirus expression system is a well established and widely used system for the production of recombinant proteins

A Trichoplusia ni derived insect cell line shown to be highly productive, in both quality and quantity, in the expression of recombinant proteins via the baculovirus expression system.

Tnao38 cells have been shown to be exceed protein expression of both Sf9 and High Five cell lines (Hashimoto et al.,2010).
Production Details Thawing Procedure: Remove cells from liquid nitrogen and thaw quickly in a 37°C water bath. Centrifuge gently (10º C, 800 rpm, 5 min) to pellet cells. Resuspend cell pellet in pre-warmed (27°C) medium and transfer to T25 flask. Grow at 27°C.
Conditional Description Tnao38 is a HighFive (Trichoplusia ni) sub-clone accidentally isolated from a primary culture of Ascalapha odarata cells
Research Area Gene Expression, Other
Growth/Phenotype Keywords Morphology: Flat fibroblast-like appearance at lower densities and more spherical appearance at higher cell densities and in suspension. Doubling time: 20.2 hrs in adherent cell culture ( see Hashimoto et al. 2010 ). Passage when ~90% confluent (Dislodge)
Recommended Growing Conditions TNMFH+10%FBS [1] in T25, T75, or T150 flasks at 27°C
Notes Absence of alphanodavirus based on RT-PCR (Hashimoto et al., 2010)

Link to original HighFive clone confirmed by SNP
Cellosaurus ID CVCL_Z252

References

There are 3 reference entries for this reagent.

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References: 3 entries

Wilde et al. 2014. Biotechnol Lett. 36(4):743-9. PMID: 24375231.

Hashimoto et al. 2012. BMC Biotechnol. 12:12. PMID: 22531032.

Hashimoto et al. 2010. BMC Biotechnol. 10:50. PMID: 20602790.


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References: 3 entries

Wilde et al. 2014. Biotechnol Lett. 36(4):743-9. PMID: 24375231.

Hashimoto et al. 2012. BMC Biotechnol. 12:12. PMID: 22531032.

Hashimoto et al. 2010. BMC Biotechnol. 10:50. PMID: 20602790.


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