Cat. #160776
Tnao38 subclone cell line
Cat. #: 160776
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Boyce Thompson Institute
Tool Details
*FOR RESEARCH USE ONLY
- Name: Tnao38 subclone cell line
- Alternate name: BTI-Tnao38
- Parental cell: High Five (T. ni)
- Growth properties: Flat fibroblast-like appearance at lower densities and more spherical appearance at higher cell densities and in suspension.
- Conditional: Yes
- Conditional description: Tnao38 is a HighFive (Trichoplusia ni) sub-clone accidentally isolated from a primary culture of? Ascalapha odarata? cells
- Description: The insect cell based baculovirus expression system is a well established and widely used system for the production of recombinant proteins A Trichoplusia ni derived insect cell line shown to be highly productive, in both quality and quantity, in the expression of recombinant proteins via the baculovirus expression system. Tnao38 cells have been shown to be exceed protein expression of both Sf9 and High Five cell lines (Hashimoto et al.,2010).Absence of alphanodavirus based on RT-PCR (Hashimoto et al., 2010)Link to original HighFive clone confirmed by SNP
- Production details: Thawing Procedure: Remove cells from liquid nitrogen and thaw quickly in a 37ÄÂ?°C water bath. Centrifuge gently (10ÄÂÄ C, 800 rpm, 5 min) to pellet cells. Resuspend cell pellet in pre-warmed (27ÄÂ?°C) medium and transfer to T25 flask. Grow at 27ÄÂ?°C.
- Cellosaurus id: CVCL_Z252
Handling
- Format: Frozen
- Growth medium: TNMFH+10S 1 in T25, T75, or T150 flasks at 27?°C
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Initial handling information: Thawing Procedure:? Remove cells from liquid nitrogen and thaw quickly in a 37?°C water bath. Centrifuge gently (10Ä C, 800 rpm, 5 min) to pellet cells. Resuspend cell pellet in pre-warmed (27?°C) medium and transfer to T25 flask. Grow at 27?°C. Doubling time: 20.2 hrs in adherent cell culture (? see Hashimoto et al. 2010? ). Passage when ~90% confluent (Dislodge)
