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Venus-A2AR vector

Invented by Luísa V Lopes
Invented at Instituto de Medicina Molecular João Lobo Antunes

Info

Catalogue Number 160728
Bacterial Resistance Amp
Selectable Markers Neomycin
Vector Type pcDNA3.1( + )
Synonyms A2AR
Antigen/Gene or Protein Targets Adenosine A2A receptor
Relevance A construct encoding a Venus-A2AR fusion protein to explore the synaptic function of A2AR
Research Area Fluorescent Cell Imaging, Neurobiology
Notes Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).

References

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References: 1 entry

Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.


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References: 1 entry

Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.


Add a reference