Instituto de Medicina Molecular João Lobo Antunes

PROP Flp reporter cell line

Invented by Sergio F de Almeida at Instituto de Medicina Molecular João Lobo Antunes

$New reporter genes allow visualisation of transcription upon DNA damage with single-molecule resolution. (A) Illustration of a HEK293 cell containing a single FRT genomic locus for single copy integration of the reporter gene. (B) Schematic of the two reporter genes with the I–SceI site and MS2/PP7 sequences highlighted. (C and D) Representative images of PROP (C) and EX2 (D) reporter cells. Images were acquired at the indicated time points after doxycycline treatment. Examples of pre-mRNAs diffusing throughout the nucleoplasm are numbered 1 to 4. The transcription site (TS) of the reporter gene is indicated in each cell. Corresponding images depicting the diffraction limited objects in the highest intensity plane are shown below. (Sci Adv. 2019 Jan 9;5(1):eaau1249. doi: 10.1126/sciadv.aau1249. eCollection 2019 Jan)$

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EX2 Flp reporter cell line

Invented by Sergio F de Almeida at Instituto de Medicina Molecular João Lobo Antunes

$New reporter genes allow visualisation of transcription upon DNA damage with single-molecule resolution. (A) Illustration of a HEK293 cell containing a single FRT genomic locus for single copy integration of the reporter gene. (B) Schematic of the two reporter genes with the I–SceI site and MS2/PP7 sequences highlighted. (C and D) Representative images of PROP (C) and EX2 (D) reporter cells. Images were acquired at the indicated time points after doxycycline treatment. Examples of pre-mRNAs diffusing throughout the nucleoplasm are numbered 1 to 4. The transcription site (TS) of the reporter gene is indicated in each cell. Corresponding images depicting the diffraction limited objects in the highest intensity plane are shown below. (Original publication: Sci Adv. 2019 Jan 9;5(1):eaau1249. doi: 10.1126/sciadv.aau1249. eCollection 2019 Jan)$