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EX2-AS Flp reporter cell line

Invented by Sergio F de Almeida
Invented at Instituto de Medicina Molecular João Lobo Antunes
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(A) Schematics of the EX2-AS reporter constructed to directly inspect break-induced antisense transcription initiation. (B) Representative images of a total of eight imaged cells and dual-line plot of a recorded cell where break-induced antisense transcription initiation was detected. (Sci Adv. 2019 Jan 9;5(1):eaau1249. doi: 10.1126/sciadv.aau1249. eCollection 2019 Jan)
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Image thumbnail for EX2-AS Flp reporter cell line
Catalogue Number 158339
Parental Line Flp-In™ T-REx™ 293 cell line
Host Human
Tissue Kidney
Model Reporter
Production Details The pOG44 Flp-recombinase expression vector was co-transfected with the EX2-antisense (AS) reporter construct into Flp-In™ T-REx™ 293 cells
Conditional No
Research Area DNA Damage and Repair, Fluorescent Cell Imaging, Genetic Studies Tools
Recommended Growing Conditions maintain under selection with 200 µg/ml Hygromycin B and 15 µg/ml Blasticidin at 37°C in a humidified atmosphere with 5% CO2.

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References: 1 entry

Vítor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.

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References: 1 entry

Vítor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.

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Inventor Information