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Caki-2 DNMT3A KO cell line

Invented by Sergio F de Almeida
Invented at Instituto de Medicina Molecular João Lobo Antunes

Info

Catalogue Number 160723
Antigen/Gene or Protein Targets DNA methyltransferase 3A
Parental Line Clear cell renal cell carcinoma cell line (Caki-2)
Synonyms TBRS, HESJAS
Host Human
Tissue Kidney
Model Cancer Model
Relevance Experimental ablation of DNMT3A gene expression using the CRISPR/Cas9 system renders Caki-2-DNMT3A knockout cells a valuable tool to investigate the role of DNA methylation in processes such as cell differentiation or cancer progression.
Production Details CRISPR/Cas9 KO
Conditional No
Research Area Cancer, DNA Damage and Repair, Epigenetics & Nuclear Signalling, Gene Expression
Recommended Growing Conditions Grow as monolayers in Dulbecco’s modified Eagle medium, supplemented with 10% (v/v) FBS, 1% (v/v) nonessential amino acids, 1% (v/v) L-glutamine and 100U/mL penicillin-streptomycin, at 37 °C in a humidified atmosphere with 5% CO2.
Notes Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.

This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.

References

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References: 1 entry

de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.


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References: 1 entry

de Matos et al. 2019. Cancers (Basel). 11(3):. PMID: 30897760.


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Inventor Information