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Monoclonal Antibodies

Anti-SARS CoV-2-Spike [3-5C-I] monoclonal antibody

Invented at LeukoCom
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Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Indicated concentration of huFc-tag antigens Sars-CoV2-Spike protein or control protein (= 0 value) were coated with 100 µl diluted in PBS onto wells of a 96 well ELISA plate for 1 h at RT. After blocking with 350 µl/well 1% BSA/PBS, the mAb 3-5C-I (100 µl/well, 3 µg/ml diluted in 1% BSA/PBS) was added over night at 4°C. After washing, HRP-coupled goat anti mouse Ig pAb (Jackson Laboratories) was incubated for 30 min at RT, subsequently washed, visualized by TMB substrate and measure by a Tecan Sunrise ELISA plate reader
Functional Enzyme-Linked Immunosorbent Assay (ELISA) 2 µg/ml recombinant ACE2 protein was coated with 100 µl diluted in PBS onto wells of a 96 well ELISA plate and incubated for 1 h at RT. After blocking with 350 µl/well 1% BSA/PBS, the recombinant SARS CoV2-spike-huFc was added (100 µl/well, 3 µg/ml diluted in 1% BSA/PBS) either alone or pre-incubated for 1 h at RT with 10 µg/ml mAb 3-5C-I and isotype matched control mAb and incubated for 2 h at RT. ACE2 coating alone and with SARS CoV2-spike-huFc incubation served as negative and positive control, subsequently. SARS CoV2-spike-huFc alone served as further negative control. After washing, HRP-coupled goat anti human Fc binding pAb (Jackson Laboratories) was incubated for 30 min at RT, subsequently washed, visualized by TMB substrate and measure by a Tecan Sunrise ELISA plate reader
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Catalogue Number 160700
Applications ELISA IHC Fn WB
Antigen/Gene or Protein Targets SARS CoV-2 Spike
Reactivity Virus
Relevance The global epidemic of novel coronavirus disease 2019 (COVID-19) was caused by a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV2). SARS-CoV2 belongs to the Beta-coronavirus family and is able to cause severe symptoms and even death with a current fatality rate of 5%. The spike protein of SAR-CoV2 plays a key role in the receptor recognition and cell membrane fusion process of the virus to host cells through it's interaction with the receptor ACE2.
Host Mouse
Immunogen Recombinant SAR-CoV-2 spike-human Fc protein
Positive Control Recombinant SARS CoV-2-Spike-human Fc protein and recombinant SARS-CoV-2 D614G Spike active Trimer HisTag protein (10587-CV-100) R&D Systems
Subclass IgG1 kappa
Myeloma Used NS1/0
Notes Purification: Protein G purified
Cross-reactivity: Wuhan strain; VOC B.1.1.7; VOC B.1351
Species reactivity: Polyhistidin-tag

This mouse monoclonal antibody recognises SARS CoV-2-spike alone or if in complex with ACE2. Consequently it can be used as a reliable diagnostic tool to detect a wide range of SARS CoV-2 strains. As it is not competing for the ACE2-SARS Cov-2-spike binding site it can interact with its viral target even if the virus is bound to its host cell.
Research Area Virology

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LeukoCom
LeukoCom

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