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Monoclonal Antibodies

Anti-pan-late cornified envelope 3, pan-LCE3 [7] monoclonal antibody

Invented by Joost Schalkwijk
Invented at Radboud UMC
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Image thumbnail for Anti-pan-late cornified envelope 3, pan-LCE3 [7] monoclonal antibody
Image thumbnail for Anti-pan-late cornified envelope 3, pan-LCE3 [7] monoclonal antibody
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Late cornified envelope (LCE)3 protein peptide recognition pattern. (a) Affinity data of anti‐LCE3 monoclonal antibody against different LCE3 proteins based on enzyme‐linked immunosorbent assay (ELISA) analysis, and comparison of the epitope sequences in the LCE3 members. The LCE3B peptide was used for mouse immunization. (b) Immunohistochemical staining of LCE3 proteins in normal human epidermis with the developed anti‐LCE3 monoclonal antibody. The picture is representative for the expression in three different skin biopsies. (c) Staining of LCE3 proteins in normal human skin after competition with our anti‐LCE3 antibody and LCE peptides. Scale bar = 100 μm.
Epidermal localization of late cornified envelope (LCE)2 and LCE3 proteins. (a) Immunofluorescence double labelling of anti‐LCE2 (red signal) and anti‐LCE3 (green signal) in normal and psoriatic skin. Nuclei are stained with 4ʹ,6‐diamidino‐2‐phenylindole (blue signal). The right panel for both skin conditions shows a magnification inlay corresponding to the white box in the left panel. (b) Immunohistochemical staining of LCE2 (upper panel) and LCE3 (lower panel) proteins in three‐dimensional skin equivalents during skin morphogenesis. Scale bar = 100 μm.
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Image thumbnail for Anti-pan-late cornified envelope 3, pan-LCE3 [7] monoclonal antibody Image thumbnail for Anti-pan-late cornified envelope 3, pan-LCE3 [7] monoclonal antibody
Catalogue Number 160445
Applications ELISA IHC IF
Antigen/Gene or Protein Targets Late cornified envelope (LCE) protein 3
Synonyms Late envelope protein 16, Small proline-rich-like epidermal differentiation complex protein 6A, Small proline-rich-like epidermal differentiation complex protein 6B
Reactivity Human
Relevance The LCE3 proteins are structural components of the cornified envelope of the stratum corneum and may be involved in innate cutaneous host defense. They ahve been shown to posses antibacterial (gram positive and negative, aerobic and anearobic speacies) properties. During inflammation they haev been shown to be involved in skin repair (PMID: 28634035).
Deletion of LCE3B and LCE3C proteins has been shown to be a significant risk factor in psoriasis.
Host Mouse
Immunogen Late cornified envelope protein 3B
Myeloma Used Sp2/0
Recommended Growing Conditions hypoxanthine-aminopterin-thymidine selection medium
Notes Although generated by using a LCE3B fragment peptide as an immunogen, this is the first pan-LCE3 monoclonal antibody. LCE3B, LCE3D and LCE3E all showed a positive ELISA signal.
Research Area Extracellular Matrix, Immunology

References

There are 1 reference entries for this reagent.

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References: 1 entry

Niehues et al. 2016. Br J Dermatol. 174(4):795-802. PMID: 26556599.

IHC IF

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References: 1 entry

Niehues et al. 2016. Br J Dermatol. 174(4):795-802. PMID: 26556599.

IHC IF

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Inventor Information

Inventors

Joost Schalkwijk

Joost Schalkwijk

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Invented at

Radboud UMC
Radboud UMC

18 reagents listed on Ximbio