Cancer Research Technology
Log in Register
Menu
Search
Recombinant Antibodies

Anti-cyanotoxin (MC-LR) scAb recombinant antibody

Invented by Caroline Murphy , Richard O'Kennedy
Invented at Dublin City University
  • Datasheet
  • Inventor Info
Print

Info

Image thumbnail for Anti-cyanotoxin (MC-LR) scAb recombinant antibody
Image thumbnail for Anti-cyanotoxin (MC-LR) scAb recombinant antibody
Add an image
Fig. 1. (A) The avian derived IgY molecule consists of four linked polypeptide chains, two heavy domains (containing one variable heavy region (VH), and four constant heavy regions (CH1, CH2, CH3 and CH4)) and two light domains (containing one variable light (VL) and one constant light (CL) region), linked by two disulphide bonds (shown in red) (Murphy et al., 2016). The antigen-binding region is composed of a variable light (VL) and variable heavy (VH) domain. The antibody is segregated into a Fragment antigen binding (Fab) and Fragment crystallizable (Fc) region. (B) Single chain fragment variable (scFv) displays structurally important complementarity determining regions (CDR), and consists of VH and VL domains linked by a serine-glycine linker. (C) The single chain antibody (scAb) fragment is composed of a human kappa light chain constant domain (CL) connected to a scFv.
Fig. 4. Competitive ELISA depicting the curves obtained from each recombinant antibody fragment (scFv and scAb). The ELISA was repeated three times and each sample point was carried out in triplicate (results represent the mean ± SD). The results were normalized by plotting the absorbance value (A) divided by the absorbance value containing no analyte (A0) to account for nonspecific binding, and are denoted on the Y-axis as A/A0.
Add an image
Image thumbnail for Anti-cyanotoxin (MC-LR) scAb recombinant antibody Image thumbnail for Anti-cyanotoxin (MC-LR) scAb recombinant antibody
Catalogue Number 158335
Applications ELISA WB
Antigen/Gene or Protein Targets Microcystin-LR
Synonyms MC-LR, Anti-Microcystin-LR (R66S) scAb
Reactivity N/A
Relevance Microcystins are cyclic heptapeptides implicated in the impairment of liver function in various animals. They have two variable amino acid residues; in the case of the cyanotoxin MC-LR the variable amino acids are Leucine (L) and Arginine (R). Microcystin-LR is the most commonly found cyanotoxin and inhibits protein phosphatase type 1 and type 2A (PP1 and PP2A) activities in the cytoplasm of liver cells, leading to hepatocytic necrosis and haemorrhaging. Chronic exposure to microcystin is a worrying phenomenon as microcystin over an extended period of time can cause cancer through the inhibition of protein phosphatases PP1 and PP2A.
Host Mouse
Immunogen Microcystin-LR
Research Area Aquaculture, Plant Science
Immunogen UniProt ID TBC
Notes IC50 : 4.5 ng mL-1
IC90 – IC10 : 0.4-52.8 ng mL-1

Storage: Shown to remain stable at room temperature (18-20oC) in the presence of 20% (v/v) glycerol. Thus can be used on site for several weeks without degradation.
Best long term storage at -20oC.

This scAb created from following scFv: Anti-Microcystin-LR (MC-LR) scFv (R66S) [R66SscFv] recombinant antibody

References

There are 1 reference entries for this reagent.

View All References

References: 1 entry

Murphy et al. 2018. J Immunol Methods. 463:127-133. PMID: 30321550.

View  

Add a reference

References: 1 entry

Murphy et al. 2018. J Immunol Methods. 463:127-133. PMID: 30321550.

View  

Add a reference

Inventor Information

Inventors

Caroline Murphy

Caroline Murphy

Richard O'Kennedy

Richard O'Kennedy

Add an inventor

Invented at

Dublin City University
Dublin City University

20 reagents listed on Ximbio