Anti-cyanotoxin (MC-LR) scAb recombinant antibody
Invented by Caroline Murphy , Richard O'Kennedy
Invented at Dublin City University
- References (1)
- Inventor Info
|Antigen/Gene or Protein Targets||Microcystin-LR|
|Synonyms||MC-LR, Anti-Microcystin-LR (R66S) scAb|
|Relevance||Microcystins are cyclic heptapeptides implicated in the impairment of liver function in various animals. They have two variable amino acid residues; in the case of the cyanotoxin MC-LR the variable amino acids are Leucine (L) and Arginine (R). Microcystin-LR is the most commonly found cyanotoxin and inhibits protein phosphatase type 1 and type 2A (PP1 and PP2A) activities in the cytoplasm of liver cells, leading to hepatocytic necrosis and haemorrhaging. Chronic exposure to microcystin is a worrying phenomenon as microcystin over an extended period of time can cause cancer through the inhibition of protein phosphatases PP1 and PP2A.|
|Research Area||Aquaculture, Plant Science|
|Immunogen UniProt ID||TBC|
IC50 : 4.5 ng mL-1
IC90 – IC10 : 0.4-52.8 ng mL-1
Storage: Shown to remain stable at room temperature (18-20oC) in the presence of 20% (v/v) glycerol. Thus can be used on site for several weeks without degradation.
Best long term storage at -20oC.
This scAb created from following scFv: Anti-Microcystin-LR (MC-LR) scFv (R66S) [R66SscFv] recombinant antibody
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