- Datasheet
- References (0)
- Inventor Info
Info
| Catalogue Number | 158050 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Interferon gamma |
| Reactivity | Salmonids |
| Relevance |
IFN-γ is a key cytokine involved in Th1 immune responses. It is mainly produced by T cells and NK cells, and drives both innate and adaptive immunity systems that promote protection against intracellular infections. IFN-γ is involved in activation of macrophages and T helper lymphocytes, stimulation of antigen presentation, control of cell proliferation and apoptosis, induction of antiviral state, immunomodulation, inflammation and leucocyte trafficking. IFN-γ is one of the most important cytokines in both innate and adaptive immune responses; therefore accurate assessment of IFN-γ levels is of critical importance in evaluating the status of the immune system. An IFN-γ antibody would be particularly useful in examining immune responses in fish to infectious agents and in the development of protective responses after vaccination. |
| Host | Rabbit |
| Immunogen | Ovalbumin-conjugated synthetic peptide. Peptide immunogen is conserved in salmonids fish species. |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 18 |
| Notes |
Applications and Recommended Usage Conditions: - ELISA recommended to be used at 1/500 dilution. Use peptide immunogen or recombinant protein as positive control. - WB recommended to be used at 1/500 dilution. Use recombinant protein as positive control. - IHC recommended to be used at 1/500 dilution. Antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal. |
| Positive Control | ELISA: peptide immunogen, recombinant protein WB: recombinant protein IHC: formalin-fixed, paraffin-embedded rainbow-trout spleen tissues |
| Research Area | Cell Type or Organelle Marker, Immunology |
References
There are 0 reference entries for this reagent.
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
Inventor Information
Inventors
|
Abdo Alnabulsi |
