Anti-(1-4)-beta-galactan [LM5] monoclonal antibody
Invented by Prof Paul Knox from PlantProbes
Invented at University of Leeds
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 157954 |
| Antigen/Gene or Protein Targets | (1[->]4)-[beta]-D-Galactan |
| Synonyms | (1-4)-beta-galactan, linear β-1,4-galactan epitope |
| Relevance | Pectins are major components of higher-plant primary cell walls. They have been shown to play an important role in cell adhesion (Vennigerholz and Walles, 1987) and are thought to carry out physiological roles by modulating cell wall pH, determining cell wall porosity, and acting as signaling molecules (Campbell and Labavitch, 1991; Carpita and Gibeaut, 1993). |
| Host | Rat |
| Immunogen | (1[->]4)-[beta]-galactotetraose-bovine serum albumin neoglycoprotein. |
| Positive Control | IgG |
| Myeloma Used | IR983F |
| Notes |
GAL4-BSA neoglycoprotein was used to immunize a set of male Wistar rats, according to standard immunization schedules. Tail bleeds were taken from each of the animals before and after immunizations, and antisera were prepared. The sera were screened for the desired antibody reactivities using an ELISA. Fusions were performed with one-half of a spleen from the rats. Lymphocytes were fused with the IR983F rat myeloma line (Bazin, 1982) based on the method of Liddel and Cryer (1991). Hybridoma supernatants from the GAL4-BSA fusion were screened for the presence of (1[->]4)-[beta]-galactan antibodies on an ELISA. A number of positive antibody-secreting cell lines were identified from the neoglycoprotein fusion. These cell lines were rescreened on an immunodot-binding assay to ascertain that the antibodies were not directed against BSA. Hybridomas were cloned by limiting dilution, and the cell lines were expanded and cryopreserved. Cell supernatants were collected and stored at 4°C. The monoclonal antibody (LM5, an IgG2c) was selected for characterization and use in further studies. |
| Research Area | Plant Science |
