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Bovine Type II Alveolar Epithelial (B2AE) cell line

Invented by Diane Lee at University of Surrey

Info

Catalogue Number 157737
Tissue Alveolus
Model Immortalised Line
Relevance Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus.
Production Details Generated by the lentiviral transduction of Bmi1 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. Proliferation rate as for primary.
Research Area Immunology, Other
Growth/Phenotype Keywords Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI)
Recommended Growing Conditions Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture ΒΌ dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2.

References

There are 3 reference entries for this reagent.

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References: 3 entries

Lee et al. 2019. Curr Protoc Toxicol. 80(1):e71. PMID: 30875462.

Lee et al. 2019. F1000Res. 8:357. PMID: 31448101.

Lee et al. 2018. Sci Rep. 8(1):11927. PMID: 30093682.


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References: 3 entries

Lee et al. 2019. Curr Protoc Toxicol. 80(1):e71. PMID: 30875462.

Lee et al. 2019. F1000Res. 8:357. PMID: 31448101.

Lee et al. 2018. Sci Rep. 8(1):11927. PMID: 30093682.


Add a reference

Inventor Information