Bovine Type II Alveolar Epithelial (B2AE) cell line
Invented by Diane Lee at University of Surrey
- Datasheet
- References (3)
- Inventor Info
Info
| Catalogue Number | 157736 |
| Tissue | Alveolus |
| Model | Immortalised Line |
| Relevance | Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus. |
| Production Details | Generated by the lentiviral transduction of SV40 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. |
| Research Area | Immunology, Other |
| Growth/Phenotype Keywords | Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI) |
| Recommended Growing Conditions | Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture ΒΌ dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2. |
References: 3 entries
Lee et al. 2019. Curr Protoc Toxicol. 80(1):e71. PMID: 30875462.
Lee et al. 2019. F1000Res. 8:357. PMID: 31448101.
Lee et al. 2018. Sci Rep. 8(1):11927. PMID: 30093682.
Add a reference
References: 3 entries
Lee et al. 2019. Curr Protoc Toxicol. 80(1):e71. PMID: 30875462.
Lee et al. 2019. F1000Res. 8:357. PMID: 31448101.
Lee et al. 2018. Sci Rep. 8(1):11927. PMID: 30093682.
Add a reference
