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Monoclonal Antibodies

Anti-uncarboxylated osteocalcin (ucOC) [Fab-13] recombinant antibody

Invented by Kaisa Ivaska-Papaioannou , Urpo Lamminmäki
Invented at University Of Turku
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Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-13] recombinant antibody
Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-13] recombinant antibody
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From Arponen et al Calcified Tissue International volume 107, pages 529–542(2020) Conversion from scFv-AP to Fab-AP improved the thermostability of the antibodies. Thermostability of the recombinant antibodies Fab-AP (open) and scFv-AP (solid) was tested by heating the antibodies Fab-AP13 (a), Fab-AP2 (b), Fab-AP16 (c), and Fab-AP19 (d) from 40 °C up to 71 °C. Reference antibody was kept on ice during the heating process and set as 100% and Tm was assessed when 50% of immunoreactivity was lost (gray dot line)
From Arponen et al Calcified Tissue International volume 107, pages 529–542(2020). Characterization of the recombinant Fab-AP–antibodies. The binding site in ucOC was determined for Fab-AP13 (black), Fab-AP2 (white), Fab-AP16 (gray), Fab-AP19 (white with stripes) and MAb-2H9 (gray with stripes) by testing biotinylated linear human osteocalcin peptides.
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Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-13] recombinant antibody Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-13] recombinant antibody
Catalogue Number 157725
Applications IF Fn
Antigen/Gene or Protein Targets osteocalcin - partially carboxylated with Glu at position 17 and 21
Synonyms Uncarboxylated osteocalcin, uc-Osteocalcin, uc-OC, bone gamma-carboxyglutamic acid-containing protein, BGLAP
Reactivity Human
Relevance Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone found in bone and dentin. Osteocalcin is a bone-specific protein which contains three glutamic acid residues (Glu) that undergo post-translational gamma-carboxylation. Uncarboxylated osteocalcin (ucOC) may participate in the regulation of glucose metabolism, thus measurement of ucOC could be useful in evaluating interactions between bone and glucose metabolism.
Immunogen Human full-length ucOC peptide (1–49, Glu at positions 17, 21 and 24; C23–C29 bridge)
Subclass IgG1 kappa
Notes Adapted from Arponen et al Calcified Tissue International volume 107, pages 529–542(2020).
These ucOC-specific recombinant antibodies were selected from an antibody library by phage display. Four candidates were characterized, and this one (Fab-AP13) was used to set up an immunoassay with a pre-existing MAb. This immunoassay (described in Arponen et al Calcified Tissue International volume 107, pages 529–542(2020)) specifically detects ucOC in human blood samples. This novel immunoassay, was characterized and found to be sensitive with LOD 0.18 ng/ml and biological sample linear down to dilution 0.8 ng/ml in serum and in plasma samples. The stability of immunoreactivity was significantly improved at + 4 °C vs room temperature and no loss of immunoreactivity was observed when samples were kept at − 20 °C. ucOC immunoreactivity was more stable in plasma than in serum. The detection of the analyte, ucOC, from biological sample depends on the antibody binding site and its stability. Fab-AP13 binds to more stable epitopes of osteocalcin.
Research Area Diabetes, Metabolism, Other

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Inventor Information

Inventors

Kaisa Ivaska-Papaioannou

Kaisa Ivaska-Papaioannou

Urpo Lamminmäki

Urpo Lamminmäki

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Invented at

University Of Turku
University Of Turku

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