Anti-uncarboxylated osteocalcin (ucOC) [Fab-2] recombinant antibody
Invented by Kaisa Ivaska-Papaioannou at University Of Turku
|Antigen/Gene or Protein Targets||osteocalcin - partially carboxylated with Glu at position 17 and 21|
|Synonyms||Uncarboxylated osteocalcin, uc-Osteocalcin, uc-OC, bone gamma-carboxyglutamic acid-containing protein, BGLAP|
|Relevance||Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone found in bone and dentin. Osteocalcin is a bone-specific protein which contains three glutamic acid residues (Glu) that undergo post-translational gamma-carboxylation. Uncarboxylated osteocalcin (ucOC) may participate in the regulation of glucose metabolism, thus measurement of ucOC could be useful in evaluating interactions between bone and glucose metabolism.|
|Immunogen||Human full-length ucOC peptide (1–49, Glu at positions 17, 21 and 24; C23–C29 bridge)|
Adapted from Arponen et al Calcified Tissue International volume 107, pages 529–542(2020).
These ucOC-specific recombinant antibodies were selected from an antibody library by phage display. Four candidates were characterized, and this one (Fab-AP13) was used to set up an immunoassay with a pre-existing MAb. This immunoassay (described in Arponen et al Calcified Tissue International volume 107, pages 529–542(2020)) specifically detects ucOC in human blood samples. This novel immunoassay, was characterized and found to be sensitive with LOD 0.18 ng/ml and biological sample linear down to dilution 0.8 ng/ml in serum and in plasma samples. The stability of immunoreactivity was significantly improved at + 4 °C vs room temperature and no loss of immunoreactivity was observed when samples were kept at − 20 °C. ucOC immunoreactivity was more stable in plasma than in serum. The detection of the analyte, ucOC, from biological sample depends on the antibody binding site and its stability. Fab-AP13 binds to more stable epitopes of osteocalcin.
|Research Area||Diabetes, Metabolism, Other|
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