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Monoclonal Antibodies

Anti-Estradiol-17-beta [S16] recombinant antibody

Invented by Urpo Lamminmäki
Invented at University Of Turku
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Image thumbnail for Anti-Estradiol-17-beta [S16] recombinant antibody
Image thumbnail for Anti-Estradiol-17-beta [S16] recombinant antibody
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SDS-PAGE gel of Anti-Estradiol-17-beta [S16] recombinant antibody in both its reduced and non-reduced state.
The Anti-Estradiol immuno complex [C6] recombinant antibody and the Anti-Estradiol-17-beta [S16] recombinant antibody were used in an immunoassay to detect E2. The EC50 of the binding was found to be 1113pg/mL.
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Catalogue Number 157720
Applications ELISA IF IP
Antigen/Gene or Protein Targets Estradiol-12-beta
Synonyms Oestradiol, E2
Reactivity Human
Relevance Estradiol is an oestrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles. With age and in the case of pathologies such as ovarian failure and following surgical removal of the oavries, levels of estradiol can fall. This results in symptoms such as osteoporosis, vaginal changes, changes to the skin and hot flushes.
Recombinant anti-estradiol immunocomplex antibody, clone [C6], binds to the immunocomplex of the anti- Estradiol-17-beta antibody clone [S16] and Estradiol-17-beta. For use in the detection of estradiol in low levels.
Subclass IgG1
Molecular Weight (kDa) 145668.74
Notes The use of this antibody alongside the anti-estradiol-immunocomplex antibody makes up the basis for an estradiol assay which addresses the very low circulatory levels of estradiol on blood samples which causes many similar competitive immunoassays to fail. Here the immunocomplex antibody is used to detect the levels of estradiol bound to anti-estradiol-17-beta, increasing the sensitivity of measurements.
Estradiol is implicated in both physiology, pathophysiology and developmental biology making this antibody pair, and the assay they facilitate, a useful tool in a number of research areas as well as for diagnostic purposes.

Please note, if this reagent will be used in a non-competitive immunoassay then you may require a license to the following patent rights which are held by VTT Technical Research Centre of Finland (US7749712B2; EP1579222B1, EP1579222B8, BRPI0316384B1, CN1711476B, JP4729701B2, NO338417B1, ZA200503475B).
Research Area Developmental Biology, Reproductive Biology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Leivo et al. 2019. Anal Bioanal Chem. 411(22):5633-5639. PMID: 31177333.

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Kokko et al. 2007. Anal Chem. 79(15):5935-40. PMID: 17602670.

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References: 2 entries

Leivo et al. 2019. Anal Bioanal Chem. 411(22):5633-5639. PMID: 31177333.

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Kokko et al. 2007. Anal Chem. 79(15):5935-40. PMID: 17602670.

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Inventor Information