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Anti-GTP [3A5] recombinant antibody
Invented by Urpo Lamminmäki at University Of Turku
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 157718 |
| Applications | ELISA FACS IF |
| Antigen/Gene or Protein Targets | GTP |
| Reactivity | Human |
| Relevance | GTPases are central cellular signaling proteins, which cycle between a GDP-bound inactive and a GTP-bound active conformation in a controlled manner. Ras GTPases are frequently mutated in cancer and so far only few experimental inhibitors exist. The most common methods for monitoring GTP hydrolysis rely on luminescent GDP- or GTP-analogs. (From Kopra et al Anal. Chem. 2015, 87, 6, 3527–3534) |
| Notes |
Recombinant GTP-specific antibody, clone GTP [2A4] which specifically detects free GTP. This potent Fab fragment (2A4(GTP)) showed over 100-fold GTP-specificity over GDP, ATP, or CTP and was used to develop a heterogeneous time-resolved luminescence based assay for the monitoring of GTP concentration. The method allows studying the GEF dependent H-Ras activation (GTP binding) and GAP-catalyzed H-Ras deactivation (GTP hydrolysis) at nanomolar protein concentrations. (From Kopra et al Anal. Chem. 2015, 87, 6, 3527–3534) |
| Research Area | Cancer, Metabolism |
References: 2 entries
Kopra et al. 2017. Anal Chem. 89(8):4508-4516. PMID: 28318223.
Kopra et al. 2015. Anal Chem. 87(6):3527-34. PMID: 25707436.
Add a reference
References: 2 entries
Kopra et al. 2017. Anal Chem. 89(8):4508-4516. PMID: 28318223.
Kopra et al. 2015. Anal Chem. 87(6):3527-34. PMID: 25707436.
Add a reference
Inventor Information
Inventors
|
Urpo Lamminmäki |
