Anti-Myeloperoxidase (MPO) scFv (B10B) [B10BscFv] recombinant antibody
Invented by Barry McDonnell , Caroline Murphy
Invented at Dublin City University
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 156520 |
| Applications | ELISA WB |
| Antigen/Gene or Protein Targets | Myeloperoxidase |
| Synonyms | MPO |
| Reactivity | N/A |
| Relevance | MPO is a mediator enzyme secreted by inflammatory cells e.g. activated neutrophils and monocytes. MPO produces hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion (Cl-) (or the equivalent from a non-chlorine halide) during the neutrophil's respiratory burst, requiring heme as a cofactor. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent (Heinecke et al., 1993). Both hypochlorous acid and tyrosyl radical are cytotoxic and are used by neutrophils to kill bacteria and other pathogens. High levels of MPO have been reported in atherosclerotic plaques (Blake and Ridker, 2003) and accumulating evidence indicates that MPO may also have a causative role in plaque vulnerability (Hazen, 2004). MPO can generate several reactive oxidised intermediates, concomitantly inducing oxidative damage of cells and tissues (Brennan et al., 2003). |
| Host | Chicken |
| Immunogen | Myeloperoxidase |
| Research Area | Cardiovascular, Other |
| Immunogen UniProt ID | TBC |
| Notes |
Affinity: 1.86x10^-10 M Application Working Concentration: ELISA (1:1,000-1:10,000) Western blotting (1:1,000-1:5,000) Storage: 1 x PBS -20C in 1 x PBS. Avoid repeated freeze and thaw cycles |
References: 1 entry
McDonnell, Barry (2010) Development of novel antibody-based diagnostics for the early and rapid detection of cardiac markers. PhD thesis, Dublin City University.
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References: 1 entry
McDonnell, Barry (2010) Development of novel antibody-based diagnostics for the early and rapid detection of cardiac markers. PhD thesis, Dublin City University.
Add a reference
Inventor Information
Inventors
|
Barry McDonnell |
|
|
Caroline Murphy |
