HaCaT CAGA12-Luc/TK Renilla cell line
Invented by Caroline Hill at The Francis Crick Institute
|Antigen/Gene or Protein Targets||Activin and transforming growth factor β (TGF-β) signalling|
|Parental Line||HEK293T cell line|
|Relevance||The human embryo kidney cell line HEK293T modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.|
Made by transfecting the human embryo kidney cell line HEK293T with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.
|Conditional Description||In the presence of activin and TGF-beta signalling luciferase is expressed.|
|Research Area||Cancer, Cell Signaling & Signal Transduction, Developmental Biology|
|Recommended Growing Conditions||Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)|
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