MDA-MB-231 CAGA12-Luc/TK Renilla cell line

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Catalogue Number	156437
Antigen/Gene or Protein Targets	Activin and transforming growth factor β (TGF-β) signalling
Parental Line	MDA-MB-231 cell line
Host	Human
Tissue	Breast
Disease Keywords	Breast cancer
Model	Reporter
Relevance	The human breast cancer cell line MDA-MB-31 modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
Production Details	Made by transfecting the breast cancer cell line MDA-MB-231 with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.Clonal
Conditional Description	In the presence of activin and TGF-beta signalling luciferase is expressed.
Research Area	Cancer, Cell Signaling & Signal Transduction, Developmental Biology
Recommended Growing Conditions	Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)+ 50 µg/ml blasticidin

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Grönroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.

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Grönroos et al. 2012. Mol Cell Biol. 32(14):2904-16. PMID: 22615489.

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Caroline Hill

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	The Francis Crick Institute
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