Anti-CD68 [Y1/82A] recombinant antibody

Invented at University of Oxford

Datasheet

References (17)

Inventor Info

Info

Catalogue Number	154814
Applications	N/A
Antigen/Gene or Protein Targets	CD68
Synonyms	CD68 Molecule; CD68 Antigen; Macrophage Antigen CD68; GP110; Scavenger Receptor Class D; Member; Scavenger Receptor Class D; SCARD1; LAMP4
Reactivity	Human
Relevance	Recombinant monoclonal antibody directed against CD68, which can aid diagnosis of monocytic leukaemia’s and histiocytic neoplasms and identify macrophages in tissues from various inflammatory and neoplastic disease states. Background and Research Application CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown. This antibody is a recombinant version of the anti-CD68 monoclonal antibody. Anti-CD68 (Y1/82A) reacted with peripheral blood and bone marrow monocytes and resident macrophages in all tissues tested. It did not react with other cell types, apart from some osteoclasts and megakaryocytes. This antibody was shown to be useful in characterising monocytic leukaemia’s, histiocytic neoplasms and detecting macrophages in disease.
Host	Mouse
Immunogen	Lysosomal contents of lung macrophages
Subclass	IgG2a
Research Area	Cell Type or Organelle Marker, Immunology, Stem Cell Biology
Immunogen UniProt ID	P34810
Notes	Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest The Y1/82A antibody recognises an epitope distinct from those of antibody clones Y2/131, EBM11, Ki-M6 and KP1. Y1/82A is reportedly more specific for monocytes and macrophages than KP1. Y1/82A bound to leukemic cells from patients with acute myelomonocytic leukaemia and acute monocytic leukaemia, but not to neoplastic cells from patients with malignant lymphoproliferative disorders or malignant epithelial tumours. Concentration 1mg/ml as standard

References

There are 17 reference entries for this reagent.

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References: 17 entries

A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.

Cañete et al. 2015. Arthritis Res Ther. 17:173. PMID: 26156866.

CD68 reactivity of non-macrophage derived tumours in cytological specimens.

Conrad et al. 2015. Arthritis Res Ther. 17:179. PMID: 26178906.

Distribution of the CD68 macrophage/myeloid associated antigen.

Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.

Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis.

In vivo pre-activation of monocytes in patients with axial spondyloarthritis.

KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.

MacParland et al. 2017. ACS Nano. :. PMID: 28040885.

Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.

Phenotype Determines Nanoparticle Uptake by Human Macrophages from Liver and Blood.

Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.

Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.

Shenoy et al. 2019. Microbiome. 7(1):37. PMID: 30857553.

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Lee et al. 2018. Sci Rep. 8(1):15076. PMID: 30305672.

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Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.

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