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Anti-CD68 [Y1/82A]
RECENTLY UPDATED

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151370
Applications
RECENTLY UPDATED
FACS IHC IF WB
Antigen/Gene or Protein Targets CD68
Synonyms CD68 Molecule; CD68 Antigen; Macrophage Antigen CD68; GP110; Scavenger Receptor Class D; Member; Scavenger Receptor Class D; SCARD1; LAMP4
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody directed against CD68, which can aid diagnosis of monocytic leukaemia’s and histiocytic neoplasms and help identify macrophages in tissues from various inflammatory and neoplastic disease states.

Background and Research Application
CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.
Anti-CD68 (Y1/82A) reacted with peripheral blood and bone marrow monocytes and resident macrophages in all tissues tested. Anti-CD68 did not react with other cell types, apart from some osteoclasts and megakaryocytes. This antibody was shown to be useful in characterising monocytic leukaemia’s, histiocytic neoplasms and detecting macrophages in disease.
Host Mouse
Immunogen Lysosomal contents of lung macrophages
Immunogen UniProt ID
RECENTLY UPDATED
P34810
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The Y1/82A antibody recognises an epitope distinct from those of antibody clones Y2/131, EBM11, Ki-M6 and KP1. Y1/82A is reportedly more specific for monocytes and macrophages than KP1.
Y1/82A bound to leukemic cells from patients with acute myelomonocytic leukaemia and acute monocytic leukaemia, but not to neoplastic cells from patients with malignant lymphoproliferative disorders or malignant epithelial tumours.

Ultroser G can be used at 1% if the cells are not growing well.

Concentration
1mg/ml as standard
Research Area
RECENTLY UPDATED
Cell Type or Organelle Marker, Immunology, Stem Cell Biology

References

There are 19 reference entries for this reagent.

View All References

References: 19 entries
RECENTLY UPDATED

Shenoy et al. 2019. Microbiome. 7(1):37. PMID: 30857553.

Lee et al. 2018. Sci Rep. 8(1):15076. PMID: 30305672.

Matossian et al. 2018. Breast Cancer Res Treat. 169(2):381-390. PMID: 29392581.

Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.

Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.

MacParland et al. 2017. ACS Nano. :. PMID: 28040885.

FACS

Phenotype Determines Nanoparticle Uptake by Human Macrophages from Liver and Blood.

Europe PMC ID: 28040885

Conrad et al. 2015. Arthritis Res Ther. 17:179. PMID: 26178906.

IF

In vivo pre-activation of monocytes in patients with axial spondyloarthritis.

Europe PMC ID: 26178906

Cañete et al. 2015. Arthritis Res Ther. 17:173. PMID: 26156866.

IF

Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis.

Europe PMC ID: 26156866

Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.

CD68 reactivity of non-macrophage derived tumours in cytological specimens.

Europe PMC ID: 7684403

Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.

Distribution of the CD68 macrophage/myeloid associated antigen.

Europe PMC ID: 2078523

Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.

A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.

Europe PMC ID: 2803980

Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.

KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.

Europe PMC ID: 2654191


Add a reference

References: 19 entries
RECENTLY UPDATED

Shenoy et al. 2019. Microbiome. 7(1):37. PMID: 30857553.

Lee et al. 2018. Sci Rep. 8(1):15076. PMID: 30305672.

Matossian et al. 2018. Breast Cancer Res Treat. 169(2):381-390. PMID: 29392581.

Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.

Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.

MacParland et al. 2017. ACS Nano. :. PMID: 28040885.

FACS

Phenotype Determines Nanoparticle Uptake by Human Macrophages from Liver and Blood.

Conrad et al. 2015. Arthritis Res Ther. 17:179. PMID: 26178906.

IF

In vivo pre-activation of monocytes in patients with axial spondyloarthritis.

Cañete et al. 2015. Arthritis Res Ther. 17:173. PMID: 26156866.

IF

Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis.

Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.

CD68 reactivity of non-macrophage derived tumours in cytological specimens.

Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.

Distribution of the CD68 macrophage/myeloid associated antigen.

Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.

A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.

Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.

KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.


Add a reference