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ZR-75-1 BCAR1 [4A12] cell line
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Catalogue Number 154621
Antigen/Gene or Protein Targets BCAR1
Parental Line ZR-75-1
Synonyms Breast Cancer Anti-Estrogen Resistance 1; CASS1; P130Cas; CRKAS
Host Human
Tissue Breast
Disease Keywords Mammary ductal carcinoma, estrogen independent
Model Cancer Model
Relevance Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype.

Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the parental (Cat No 154547).

This cell line is a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
Production Details Full length BCAR1/p130Cas cDNA was introduced in the estrogen-dependent ZR-75-1 cell line by transfection
Research Area Cancer, Drug Discovery & Development
Recommended Growing Conditions RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17β-estradiol
Notes Murine BCAR1 cDNA is homologus to the human p130Cas
The cell line is resistant to Geneticin, which may be included in the culture medium to ensure that the expression vector is retained by the cells.
As a consequence of the presence of a BCAR genes, these cells can also proliferate in the absence of estrogen or even in the presence of anti-estrogens.

References

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References: 1 entry

Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.


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References: 1 entry

Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.


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