ZR-75-1 BCAR1 [4A12] cell line

Tool Details

*FOR RESEARCH USE ONLY

• Name: ZR-75-1 BCAR1 [4A12] cell line
• Alternate name: Breast Cancer Anti-Estrogen Resistance 1; CASS1; P13Cas; CRKAS
• Tool sub type: Continuous
• Parental cell: ZR-75-1
• Organism: Human
• Tissue: Breast
• Disease: Cancer
• Model: Cancer Model
• Description: Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into an estrogen-independent and therapy-resistant phenotype. Seven genes including AKT1, AKT2, BCAR1, BCAR2, BCAR3, EGFR2 and GRB7 have been shown to directly underlie estrogen independence in human breast cancer cells. This cell line is part of a panel of 16 cell lines (Cat No 154621-154635, 154642) which have been transfected with these genes, plus the pare...
• Production details: Full length BCAR1/p130Cas cDNA was introduced in the estrogen-dependent ZR-75-1 cell line by transfection
• Biosafety level: 1
• Additional notes: Murine BCAR1 cDNA is homologus to the human p130Cas The cell line is resistant to Geneticin, which may be included in the culture medium to ensure that the expression vector is retained by the cells. As a consequence of the presence of a BCAR genes, these cells can also proliferate in the absence of estrogen or even in the presence of anti-estrogens.

Target Details

• Target: BCAR1

Applications

• Application notes: Murine BCAR1 cDNA is homologus to the human p130Cas

Handling

• Format: Frozen
• Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine serum (RBCS) and 1 nM 17?-estradiol
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• Brinkman et al. 2000. J Natl Cancer Inst. 92(2):112-20. PMID: 10639512.