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Catalogue Number
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154620 |
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Parental Line
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ZR-75-1
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Host
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Human
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Tissue
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Breast
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Disease Keywords
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Mammary ductal carcinoma, estrogen-dependant
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Model
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Cancer Model
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Relevance
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Hygromycin-B-resistant variant of ZR-75-1 human breast-cancer cells
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Production Details
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ZR-75-1 cells were transfected with the expression construct PGK-Hyg Br which confers hygromycin B resistance using cationic liposome. Stable transfectants were selected with 60ug/ml of hygromycin B in RBCS medium supplemented with 1nM estradiol.
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Research Area
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Cancer
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Recommended Growing Conditions
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RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS) and 1 nM 17β-estradiol
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Notes
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These cells were used as acceptor cells for somatic cell fusion experiments
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