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ZR-75-1 HygroR [ZH3D7] cell line
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Catalogue Number 154620
Parental Line ZR-75-1
Host Human
Tissue Breast
Disease Keywords Mammary ductal carcinoma, estrogen-dependant
Model Cancer Model
Relevance Hygromycin-B-resistant variant of ZR-75-1 human breast-cancer cells
Production Details ZR-75-1 cells were transfected with the expression construct PGK-Hyg Br which confers hygromycin B resistance using cationic liposome. Stable transfectants were selected with 60ug/ml of hygromycin B in RBCS medium supplemented with 1nM estradiol.
Research Area Cancer
Recommended Growing Conditions RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS) and 1 nM 17β-estradiol
Notes These cells were used as acceptor cells for somatic cell fusion experiments

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References: 1 entry

Dorssers et al. 1997. Int J Cancer. 72(4):700-5. PMID: 9259413.


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References: 1 entry

Dorssers et al. 1997. Int J Cancer. 72(4):700-5. PMID: 9259413.


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