Cat. #154620
ZR-75-1 HygroR [ZH3D7] cell line
Cat. #: 154620
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Cancer Model
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lambert Dorssers
Institute: Erasmus University Medical Center (Erasmus MC)
Tool Details
*FOR RESEARCH USE ONLY
- Name: ZR-75-1 HygroR [ZH3D7] cell line
- Tool sub type: Continuous
- Parental cell: ZR-75-1
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Model: Cancer Model
- Description: Hygromycin-B-resistant variant of ZR-75-1 human breast-cancer cells. These cells were used as acceptor cells for somatic cell fusion experiments
- Production details: ZR-75-1 cells were transfected with the expression construct PGK-Hyg Br which confers hygromycin B resistance using cationic liposome. Stable transfectants were selected with 60ug/ml of hygromycin B in RBCS medium supplemented with 1nM estradiol.
- Biosafety level: 1
- Additional notes: These cells were used as acceptor cells for somatic cell fusion experiments
Applications
- Application notes: The cell line is resistant to Geneticin, which may be included in the culture medium to ensure that the expression vector is retained by the cells.
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated bovine calf serum (RBCS) and 1 nM 17?-estradiol
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Dorssers et al. 1997. Int J Cancer. 72(4):700-5. PMID: 9259413.
