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Small Molecules (Tool Compounds)

Glycated HSA Fluorecein Boronic Acid small molecule (tool compound)

Invented by Marta P. Pereira Morais
Invented at University Of Bath
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Image thumbnail for Glycated HSA Fluorecein Boronic Acid small molecule (tool compound)
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Flu-PAGE analysis of in vitro glycated serum. SDS-PAGE of human serum glycated with 50 mMsugar at 37uC for 7 and 10 days, lanes 2–7 and 8–12 respectively. Shown are non-incubated control (lane 1) and samples incubated with glucose (lanes 2 and 8), fructose (lanes 3 and 9), mannose (lanes 4 and 10), maltose (lanes 5 and 11), galactose (lanes 6 and 12), sucrose (lane 7). The left panel shows Coomassie stained gel (samples 1–12) and the right panel shows the gel visualised with UV prior to staining (lanes 19–129). The arrows indicate fluorescent proteins identified by MS.
Flu-PAGE analysis of TASTPM mouse cortex homogenates. Flu-PAGE gel profile of TASTPM cortex homogenates, visualised with Dark ReaderH (right panel, lanes 29 and 39) prior to Coomassie staining (left panel, lanes 1–3). The solid arrows indicate the positions of the proteins that have been identified using MALDI-TOF analysis. The dotted arrows represent higher molecular weight fluorescent proteins to be investigated.
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Catalogue Number 154473
Antigen/Gene or Protein Targets Advanced Glycation Endproducts (AGE); specifically Glycated Human Serum Albumin (HSA). Also binds ApoA-I protein
Synonyms AGEs
Relevance Protein glycation, also known as non-enzymatic glycosylation, has been implicated in various disease states and is therefore an important biomarker for ageing and age-related chronic diseases such as diabetes, cardiovascular diseases, autoimmune diseases, cancer, and Alzheimer’s disease.

However their analysis is challenging due to the complexity of the protein-carbohydrate adducts. Fluorescent boronic acids like these enable the detection and identification of individual glycated proteins in complex samples through gel electrophoresis.
Research Area Neurobiology, Protein Degradation, Cardiovascular, Immunology
Notes This small molecule has been used to identify glycated proteins in human serum, insect hemolymph, and mouse brain homogenates.

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References: 1 entry

Pereira Morais et al. 2013. Sci Rep. 3:1437. PMID: 23531746.

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References: 1 entry

Pereira Morais et al. 2013. Sci Rep. 3:1437. PMID: 23531746.

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Inventor Information

Inventors

Marta P. Pereira Morais

Marta P. Pereira Morais

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Invented at

University Of Bath
University Of Bath

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