16E6/E7 HCK cell line
Invented by Prof Aloysius Klingelhutz from The University Of Iowa
Invented at The University Of Iowa
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154442 |
| Antigen/Gene or Protein Targets | Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies |
| Parental Line | Human ectocervix cells |
| Host | Human |
| Tissue | Cervix |
| Relevance | Expresses cervical epithelia cell markers; responds to inflammatory agents; supports growth of cervical pathogens |
| Production Details | The HPV16 E6/E7 open reading frames were cloned into a murine-based retroviral vector, LXSN, which contains the neomycin resistance gene. The constructs were transfected into the packaging celline PA317, and recombinant retroviruses in the supernatant were collected. Early-passage cx cells were infected at a high titer with the different retroviral constructs, plated at various densities, selected in G418, and then passaged as pools or ring cloned. Eight ring clones were isolated for each group. Cells were passaged 1:5 when confluent. |
| Research Area | Cancer, Microbiology, Virology |
| Growth/Phenotype Keywords | E6/E7-immortalized cells generally showed an increase in telomere length as they were passaged in culture, with some later passage lines having telomeres that were similar to or longer than the earliest-passage precrisis cells examined. |
| Recommended Growing Conditions | GIBCO keratinocyte serum-free media. G418 selection. |
| Notes | Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies. |
References: 2 entries
Klingelhutz et al. 1994. Mol Cell Biol. 14(2):961-9. PMID: 8289836.
Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.
Europe PMC ID: 8289836
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References: 2 entries
Klingelhutz et al. 1994. Mol Cell Biol. 14(2):961-9. PMID: 8289836.
Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.
Add a reference
