16E6/E7 HCK cell line

Tool Details

*FOR RESEARCH USE ONLY

• Name: 16E6/E7 HCK cell line
• Tool sub type: Continuous
• Parental cell: Human ectocervix cells
• Organism: Human
• Tissue: Cervix
• Growth properties: E6/E7-immortalized cells generally showed an increase in telomere length as they were passaged in culture, with some later passage lines having telomeres that were similar to or longer than the earliest-passage precrisis cells examined.
• Description: Expresses cervical epithelia cell markers; responds to inflammatory agents; supports growth of cervical pathogens. Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.
• Application: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.
• Production details: The HPV16 E6/E7 open reading frames were cloned into a murine-based retroviral vector, LXSN, which contains the neomycin resistance gene. The constructs were transfected into the packaging celline PA317, and recombinant retroviruses in the supernatant were collected. Early-passage cx cells were infected at a high titer with the different retroviral constructs, plated at various densities, selected in G418, and then passaged as pools or ring cloned. Eight ring clones were isolated for each gro...
• Biosafety level: 1

Target Details

• Target: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies

Applications

• Application: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies.

Handling

• Format: Frozen
• Growth medium: GIBCO keratinocyte serum-free media. G418 selection.
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• Klingelhutz et al. 1994. Mol Cell Biol. 14(2):961-9. PMID: 8289836.
• Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells.