Siglec-F KO mouse
Invented at University of Dundee
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154182 |
| Antigen/Gene or Protein Targets | Siglec-F |
| Synonyms | Siglec-8, Sialic Acid Binding Ig Like Lectin 8, SAF-2, |
| Model | Knock-Out |
| Relevance | Siglecs-8 and -F are paralogous membrane proteins highly expressed on human and mouse eosinophils respectively. They are members of the CD33-related sialic acid binding Ig-like lectin family and contain immunoreceptor tyrosine based inhibitory motifs (ITIM) and ITIM-like motifs in their cytoplasmic tails that are implicated in negative regulatory functions |
| Production Details | Siglec FR114D knock in mice, carrying an Arg114 to Asp mutation in the Siglec F gene predicted to abolish sialic acid binding were generated by Taconic Artemis using C57Bl/6 ES cells (targeting strategy illustrated in PMID: 24698729). Further crossing of homozygous Siglec FR114D mice with Tg (Nes-Cre)1Wme/J(Bal1-Cre) mice produced offspring with mosaicism/partial deletion of exons 6-9 in adult organs (including germline) resulting in the truncated Siglec F knock out allele. Mosaic offspring were then used to generate Siglec F KO mice |
| Conditional | No |
| Strain | C57BL/6 |
| Zygosity | Homozygous |
| Research Area | Adhesion, Immunology |
References: 2 entries
McMillan et al. 2014. Immunol Lett. 160(1):11-16. PMID: 24698729.
Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.
Europe PMC ID: 24698729
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References: 2 entries
McMillan et al. 2014. Immunol Lett. 160(1):11-16. PMID: 24698729.
Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.
Add a reference
