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Immortalised Human Melanocyte [PIG1] Cell Line

Invented by Professor Pranab K Das

Info

Catalogue Number 154099
Parental Line Human foreskin
Host Human
Model Immortalised Line
Relevance Melanocytes are the cell of origin for a number of conditions including melanoma an aggressive type of skin cancer and vitiligo a long-term condition characterised by patches of the skin losing their pigment. Melanoma is the most dangerous type of skin cancer. Globally, in 2012, it newly occurred in 232,000 people. In 2015 there were 3.1 million with active disease which resulted in 59,800 deaths. Australia and New Zealand have the highest rates of melanoma in the world. While Vitiligo affects 1% of the worlds population with no known cause. Immortalised melanocytes can be used as a tool for research into different melanocytic lineages
Production Details Melanocyte cultures were establised from neonatal foreskin using standard methods. Melanocytes from passage 12 were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In addition the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
Conditional No
Research Area Cancer, Cell Type or Organelle Marker
Growth/Phenotype Keywords Cells were routinely passaged 1:4 at confluency
Recommended Growing Conditions Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100µg/ml streptomycin

References

There are 4 reference entries for this reagent.

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References: 4 entries

Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937.

Immortalization of human melanocytes does not alter the de novo properties of nitric oxide to induce cell detachment from extracellular matrix components via cGMP.

Europe PMC ID: 18594937

Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834.

Generation of a human melanocyte cell line by introduction of HPV16 E6 and E7 genes.

Europe PMC ID: 9028834


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References: 4 entries

Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937.

Immortalization of human melanocytes does not alter the de novo properties of nitric oxide to induce cell detachment from extracellular matrix components via cGMP.

Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834.

Generation of a human melanocyte cell line by introduction of HPV16 E6 and E7 genes.


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Inventor Information

Inventors

Professor Pranab K Das

Professor Pranab K Das

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