Cat. #154099
Immortalised Human Melanocyte [PIG1] Cell Line
Cat. #: 154099
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Tissue: Neonatal foreskin
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pranab K Das
Institute: Amsterdam University
Primary Citation: Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834
Tool Details
*FOR RESEARCH USE ONLY
- Name: Immortalised Human Melanocyte [PIG1] Cell Line
- Tool sub type: Continuous
- Parental cell: Human foreskin
- Organism: Human
- Gender: Male
- Tissue: Neonatal foreskin
- Growth properties: Cells were routinely passaged 1:4 at confluency
- Model: Immortalised Line
- Conditional: No
- Description: Immortalized human melanocyte cell line established by introduction of retroviral construct carrying a geneticin resistance gene and containing HPV16E6E7 open reading frames
- Application: Melanocyte biology research, in vitro studies on the etiology of pigmentary disorders and melanoma
- Production details: Melanocyte cultures were established from neonatal foreskin using standard methods. Melanocytes from passage 12 were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In addition the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
- Biosafety level: 1
- Cellosaurus id: CVCL_S410
Applications
- Application: Melanocyte biology research, in vitro studies on the etiology of pigmentary disorders and melanoma
Handling
- Format: Frozen
- Volume: 1 ml
- Growth medium: Ham's F10 medium supplemented with 10ng/ml tetradecanoly phorbol 13-acetate (TPA), 0.1mM 3-isobutyl-methyl-xanthine (IBMX), 1% vol/vol Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100 ug/ml streptomycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Subculture routine: Cells were routinely passaged 1:4 at confluency
- Cultured in antibiotics: Penicillin / Streptomycin
- Mycoplasma free: Yes
- Characterisation tests: E6E7 expression, proliferation rate, anchorage independent growth, expression of melanoma markers, melanin synthesis, and ploidy
References
- Ivanova et al. 2008. In Vitro Cell Dev Biol Anim. 44(8-9):385-95. PMID: 18594937
- Le Poole et al. 1997. In Vitro Cell Dev Biol Anim. 33(1):42-9. PMID: 9028834
