CaMPARI 2.0 plasmids
Invented at Howard Hughes Medical Institute
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 154044 |
| Relevance | CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. CaMPARI 2.0 is the second generation of CaMPARI molecules with improve green brightness by 50% and red brightness by 250%. Additionally, CaMPARI 2.0 has faster kinetics, and a lower photoconversion rate in low calcium conditions resulting in a net 100-fold difference in green-to-red switching in low- vs. high-calcium conditions. Lastly, these different CaMPARI proteins display a range of sensitivity in their calcium binding (Kd’s ranging from 100 nM to 1 uM of free calcium). |
| Research Area | Fluorescent Cell Imaging, Neurobiology |
References: 2 entries
Improved methods for marking active neuron populations.
Europe PMC ID: 30361563
Moeyaert et al. 2018. Nat Commun. 9(1):4440. PMID: 30361563.
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References: 2 entries
Improved methods for marking active neuron populations.
Moeyaert et al. 2018. Nat Commun. 9(1):4440. PMID: 30361563.
Add a reference
