Cat. #154044
CaMPARI 2.0 plasmids
Cat. #: 154044
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Contributor
Institute: Howard Hughes Medical Institute
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: CaMPARI 2.0 plasmids
- Description: CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. CaMPARI 2.0 is the second generation of CaMPARI molecules with improve green brightness by 50% and red brightness by 250%. Additionally, CaMPARI 2.0 has faster kinetics, and a lower photoconversion rate in low calcium conditions resulting in a net 100-fold difference in green-to-red switching in low- vs. high-calcium conditions. Lastly, these different CaMPARI proteins display a range of sensitivity in their calcium binding (Kd’s ranging from 100 nM to 1 uM of free calcium).
- Additional notes: CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time. CaMPARI 2.0 is the second generation of CaMPARI molecules with improve green brightness by 50% and red brightness by 250%. Additionally, CaMPARI 2.0 has faster kinetics, and a lower photoconversion rate in low calcium conditions resulting in a net 100-fold difference in green-to-red switching in low- vs. high-calcium conditions. Lastly, these different CaMPARI proteins display a range of sensitivity in their calcium binding (Kd’s ranging from 100 nM to 1 uM of free calcium).
References
- Improved methods for marking active neuron populations.
- Moeyaert et al. 2018. Nat Commun. 9(1):4440. PMID: 30361563.
