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Monoclonal Antibodies

Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody

Invented by Dr Bernhard B. Singer from LeukoCom
Invented at LeukoCom
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Image thumbnail for Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody
Image thumbnail for Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody
Image thumbnail for Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody
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Flow cytometry histogram: CHO-huCEACAM5 and CHO-MacCEACAM5 transfectants were stained with purified mAb ivi4/27 (5 µg/ml; thick line) or isotype matched control mAb (thin line), followed by goat anti-mouse-FITC and analyzed by flow cytometer. Dead cells were discriminated by staining with propidium iodide (PI) and excluded from the analysis.
Flow Cytometry: Indicated CEACAM transfectants were stained with purified mAb ivi 4/27 (5 µg/ml) followed by goat anti-mouse-FITC and analyzed by flow cytometer. Dead cells were discriminated by staining with propidium iodide (PI) and excluded from the analysis.
Indirect Enzyme-Linked Immunosorbent Assay (ELISA): Indicated antigens were coated onto wells. After blocking with 2% BSA/PBS mAb ivi 4/27 (5 µg/ml) was incubated with the antigens. After washing HRP-coupled goat anti mouse antibody was incubated and visualized by TMB detection.
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Image thumbnail for Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody Image thumbnail for Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody Image thumbnail for Anti-CEACAM5/6/8 (CD66e/c/b) [ivi4/27] monoclonal antibody
Catalogue Number 154005
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets human CEACAM 5, 6, and 8; macaque CEACAM 5
Reactivity Human
Host Mouse
Immunogen Human CEA derivative; in vitro immunisation
Subclass IgG1 kappa
Myeloma Used NS1/0
Notes CEACAMs (Carcinoembryonic antigen-related (CEA) cell adhesion molecules) are highly glycosylated membrane anchored proteins. CEACAM genes make up the CEA family belonging to the immunoglobulin superfamily. CEACAMs are involved in cell adhesion and cell-cell communication processes. CEACAM 5 and 6 but NOT CEACAM8 serve as pathogen receptors (e.g. for Helicobacter pylori, Neisseria, Moraxella, Candida albicans). Immunologically they are characterized as members of the CD66 cluster of differentiation.

Antibodies to CEACAMs are commonly used in immunohistochemistry and flow cytometry to identify cells expressing the glycoproteins. However, CEACAM 5 and 6 are also found in serum where they can serve as tumor markers. In adults CEACAM5 and 6 are expressed in epithelia and CEACAM6 and 8 in granulocytes.
Research Area Cancer, Immunology

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