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NS0 Cell Line

Info

Catalogue Number 153797
Parental Line NS1 (P3/NS1/1-Ag4-1)
Host Mouse
Disease Keywords Murine myeloma
Relevance A non-secreting cell line used to generate antibody secreting hybridoma cell lines.

Background and Research Application
NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1). The cells are resistant to 10µM azaguanine, cells die in the presence of HAT medium. NS0 is widely used as a fusion partner to generate antibody secreting hybridoma cell lines and is also used as a model B-cell line.

The NS0 cell line is the property of MRC. Ximbio is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from MRC, negotiated by LifeArc (formerly known as MRC Technology); requests for commercial use of this cell line will be forwarded to LifeArc.
Research Area Other
Growth/Phenotype Keywords Suspension
Recommended Growing Conditions Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Notes Points of Interest

NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.

Concentration
Vial has between 1-5 million cells as standard, however this may vary.
Cellosaurus ID CVCL_3940

References

There are 9 reference entries for this reagent.

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References: 9 entries

Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278.

Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119.

Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743.

Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796.

Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592.

Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347.

Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818.

Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921.

Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683.


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References: 9 entries

Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278.

Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119.

Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743.

Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796.

Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592.

Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347.

Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818.

Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921.

Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683.


Add a reference

Inventor Information

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