Cancer Research Technology
Log in Register
Menu

NS0 Cell Line

Info

Catalogue Number 153797
Parental Line NS1 (P3/NS1/1-Ag4-1)
Host Mouse
Disease Keywords Murine myeloma
Relevance A non-secreting cell line used to generate antibody secreting hybridoma cell lines.

Background and Research Application
NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1). The cells are resistant to 10µM azaguanine, cells die in the presence of HAT medium. NS0 is widely used as a fusion partner to generate antibody secreting hybridoma cell lines and is also used as a model B-cell line.

The NS0 cell line is the property of MRC. Ximbio is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from MRC, negotiated by LifeArc (formerly known as MRC Technology); requests for commercial use of this cell line will be forwarded to LifeArc.
Research Area Other
Growth/Phenotype Keywords Suspension
Recommended Growing Conditions Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Notes Points of Interest
For RUO or IVD only and not for resale.
NS0 Cell Line from murine myeloma has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.

Concentration
Vial has between 1-5 million cells as standard, however this may vary.
Cellosaurus ID CVCL_3940

References

There are 9 reference entries for this reagent.

View All References

References: 9 entries

Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278.

Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119.

Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743.

Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796.

Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592.

Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347.

Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818.

Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921.

Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683.


Add a reference

References: 9 entries

Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278.

Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119.

Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743.

Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796.

Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592.

Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347.

Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818.

Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921.

Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor