Cat. #153797
NS0 Cell Line
Cat. #: 153797
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Mouse
Disease: Murine myeloma
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Medical Research Council
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: NS0 Cell Line
- Parental cell: NS1 (P3/NS1/1-Ag4-1)
- Organism: Mouse
- Disease: Murine myeloma
- Growth properties: Suspension
- Description: NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1 (P3/NS1/1-Ag4-1). The cells are resistant to 10µM azaguanine, cells die in the presence of HAT medium. NS0 is widely used as a fusion partner to generate antibody secreting hybridoma cell lines and is also used as a model B-cell line. The NS0 cell line is the property of MRC. CancerTools.org is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from MRC, negotiated by LifeArc (formerly known as MRC Technology); requests for commercial use of this cell line will be forwarded to LifeArc. The NS0 Cell Line has been used to culture NS0 cells expressing CD19scFv (a binding domain of B-lymphocyte antigen CD19) to screen hybridoma clones.
- Cellosaurus id: CVCL_3940
Handling
- Format: Frozen
- Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Temperature: 37.0°C ± 1.0°C
- Atmosphere: 5.0% ± 1.0% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Maintain cultures between 3-9x100,000 cells/mL. Cells can adhere loosely to the culture vessel surface; remove by tapping against the flask. Cultures that are more than 2 days old may have to be scraped off. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
- Mycoplasma free: Yes
References
- Lau et al. 2020. MAbs. 12(1):1686319. PMID: 31671278
- Low et al. 2020. MAbs. 12(1):1709322. PMID: 31924119
- Challa et al. 2019. MAbs. 11(5):848-860. PMID: 30964743
- Cao et al. 2019. MAbs. 11(3):489-499. PMID: 30786796
- Loebrich et al. 2019. MAbs. 11(2):335-349. PMID: 30252592
- Wada et al. 2019. MAbs. 11(2):350-372. PMID: 30466347
- Sasso et al. 2018. MAbs. 10(5):730-737. PMID: 29658818
- Bradbury et al. 2018. MAbs. 10(4):539-546. PMID: 29485921
- Galfrè et al. 1981. Methods Enzymol. 73(Pt B):3-46. PMID: 7300683
