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Human AGS NOD1 KO (Clone 41H8) Cell Line

Info

Catalogue Number 153734
Antigen/Gene or Protein Targets Human nucleotide-binding oligomerization domain-containing protein 1
Parental Line AGS
Synonyms Human nucleotide-binding oligomerization domain-containing protein 1, NOD1
Host Human
Tissue Gastric
Disease Keywords Gastric Adenocarcinoma, Helicobacter pylori infection
Model Knock-Out
Relevance NOD1 responds to Helicobacter pylori infection in a Type IV secretion system-dependent manner. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 (as generated by CRISPR) can be used to model gastric adenocarcinoma in the context of various pathogens. NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells.
Production Details CRISPR/Cas9 gene editing was used to generate NOD1 knock out clones. AGS cells were transfected with vectors expressing “Cas9 nickase” nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. Clone 41H8 was confirmed as having a 31 base pair deletion within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1.
Conditional No
Research Area Cancer, Cell Signaling & Signal Transduction, Immunology
Growth/Phenotype Keywords Epithelial-like morphology
Recommended Growing Conditions RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
Positive Control NOD1 Ligand: C12-iE-DAP (10 ng/ml), Human AGS Control Cell Line
Notes Recommended Freezing Composition: 90% (v/v) FCS - 10% (v/v) DMSO

CRISPR edited Human AGS cells.
Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.

This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.

The control Human AGS Control Cell Line can be purchased from ATCC http://www.lgcstandards-atcc.org/products/all/CRL-1739.aspx?geo_country=gb.

References

There are 2 reference entries for this reagent.

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References: 2 entries

Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.

NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.

Europe PMC ID: 29392836


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References: 2 entries

Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.

NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.


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Inventor Information

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