Anti-Myelin Basic Protein [MBP40]

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Clone 12 used to detect MBP in myelinating cell cultures using Immunofluorescence. Time-lapse imaging of the putative assembly of myelin membrane. B) The cells from the Petri dish imaged with confocal microscopy were immunostained with anti-GFP and anti-MBP to confirm differentiation of cyto-GFP labelled oligodendrocytes. Source: Ioannidou et al. 2012. PLoS One. 7(1):e30775. PMID: 22303455.

Clone 12 used to detect MBP in myelinating cell cultures using Immunofluorescence. Immunohistochemistry of transplanted neurospheres demonstrate that cyto-GFP labelled cells form early and mature myelinating oligodendrocytes.Cyto-GFP-expressing neurospheres were transplanted into a shiverer mouse 3, 7 or 15 days post-transplantation, and 10 µm thick frozen sections were cut and immunolabelled with antibodies to GFP and MBP. Source: Ioannidou et al. 2012. PLoS One. 7(1):e30775. PMID: 22303455.

Clone MBP 12 used to detect myelinated structures in MS plaques by IHC-P. Serial sections of paraffin-embedded MS tissue immunostained with anti-EP(A), clone26(B), clone2(C), clone14(D), clone12(E), or clone22(F) .Notice that only abnormal myelin tissues strongly stained by anti-EP, whereas all other antibodies strongly stain the normal myelin surrounding the plaque area. See Materials and Methods for details. Source: Matsuo et al. 1997. Am J Pathol. 150(4):1253-66. PMID: 9094982.

Clone 12 used to detect expression of MBP in mouse brain lysates via Western Blot and ICC. ...A. MBP protein can only be detected by immunocytochemistry in differentiated Schwann cells B, Western Blots of undifferentiated and differentiated primary Schwann cells show MBP protein only present in differentiated Schwann cells C, MBP and sncRNA715-specific RT-PCR on RNA extracted from undifferentiated or differentiated primary Schwann cells. Source: Müller et al. 2015. PLoS One. 10(8):e0136900. PMID: 26317513.

Clone 12 used to detect MBP in mouse oligodendrocytes using Immunofluorescence and Western Blot. α-Syn impairs oligodendrocyte maturation. Oligodendrocyte progenitor cells were either untreated (Co) or incubated with rh α-Syn (10 μg/ml) 2 h after plating for 3 or 6 days. Cells were subjected to immunocytochemistry using antibodies: a anti-acetylated α-tubulin (green) and anti-MBP (red); b anti-proteoglycan NG-2 (green) and anti-MBP (red). Nuclei were stained with DAPI (blue). Scale bar: 20 μm. c Exogenously applied α-Syn led to an increase in NG-2 and a decrease in MBP levels. Source: Grigoletto et al. 2017. Acta Neuropathol Commun. 5(1):37. PMID: 28482862.

Clone 12 used to detect expression of MBP in mouse brain lysates via Western Blot. B, Western Blots of lysates from P18 mouse brain (brain lysate), primary oligodendrocytes (pOL, 7DIV), IMS32 and Oli-neu cells using MBP and GAPDH (loading control) specific antibodies. Source: Müller et al. 2015. PLoS One. 10(8):e0136900. PMID: 26317513.

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Catalogue Number	153643
Applications	ELISA
Antigen/Gene or Protein Targets	Myelin Basic Protein
Synonyms	Myelin basic protein, MBP, 20 kDa microtubule-stabilizing protein, Myelin A1 protein
Reactivity	Chicken, Human, Guinea Pig, Pig, Rat, Rabbit, Sheep and Mammals
Relevance	Monoclonal antibody used to detect MBP levels and myelination. Background and Research Application Myelin Basic Protein (MBP) is involved in the process of myelination of nerves, oligodendrocytes and Schwann cells in the nervous system. MBP maintains the correct structure of myelin, interacting with the lipids in the myelin membrane. MBP clone 40 is used in clinical diagnostics to detect MBP levels or myelination in human MBP. MBP 40 is used in a two-site ELISA with clone MBP 12. Both are used interchangeably as capture and detection antibody.
Host	Rat
Immunogen	Epitope unknown
Immunogen UniProt ID	P02686
Positive Control	Brain tissue
Subclass	IgG2a
Molecular Weight (kDa)	13-21
Myeloma Used	NS0
Notes	Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Suggested dilutions for use Western Blot 1:100-1:2,000 Immunohistochemistry 1:10-1:500 ELISA 1:100-1:2,000 Immunofluorescence 1:10-1:500 Immunocytochemistry 1:5-1:1000 Research Areas: Neurobiology; Cell Type or Organelle Marker; Immunology; Genetics; Pathology; Stem Cell Biology Concentration 1mg/ml as standard
Research Area	Cell Type or Organelle Marker, Immunology, Neurobiology, Stem Cell Biology

References

There are 49 reference entries for this reagent.

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References: 49 entries

Grigoletto et al. 2017. Acta Neuropathol Commun. 5(1):37. PMID: 28482862.

View

Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss.

Europe PMC ID: 28482862

View

Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.

View

Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.

View

Fernandes et al. 2016. J Control Release. 238:300-310. PMID: 27369863.

View

Isoda et al. 2016. Neurosci Res. 110:18-28. PMID: 27083781.

View

Lim et al. 2016. Antioxidants (Basel). 5(3):. PMID: 27618111.

View

Protandim Protects Oligodendrocytes against an Oxidative Insult.

Europe PMC ID: 27618111

View

Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.

Europe PMC ID: 27417499

View

Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology.

Europe PMC ID: 27369863

View

Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions.

Europe PMC ID: 27083781

View

Crawford et al. 2016. Am J Pathol. 186(3):511-6. PMID: 26773350.

View

Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination.

Europe PMC ID: 26773350

View

Natrajan et al. 2015. Brain. 138(Pt 12):3581-97. PMID: 26463675.

View

Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.

Europe PMC ID: 26463675

View

HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.

Europe PMC ID: 26406915

View

SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.

Europe PMC ID: 26317513

View

Meade et al. 2015. Brain Res. 1611:101-13. PMID: 25842371.

View

Quantitative proteomic analysis of the brainstem following lethal sarin exposure.

Europe PMC ID: 25842371

View

Brügger et al. 2015. PLoS Biol. 13(9):e1002258. PMID: 26406915.

View

Müller et al. 2015. PLoS One. 10(8):e0136900. PMID: 26317513.

View

Pusic et al. 2014. J Neuroimmunol. 266(1-2):12-23. PMID: 24275061.

View

IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.

Europe PMC ID: 24275061

View

Horiuchi et al. 2012. Neurobiol Aging. 33(3):499-509. PMID: 20594620.

View

Time-lapse imaging of the dynamics of CNS glial-axonal interactions in vitro and ex vivo.

Europe PMC ID: 22303455

View

Ioannidou et al. 2012. PLoS One. 7(1):e30775. PMID: 22303455.

View

Monk et al. 2011. Development. 138(13):2673-80. PMID: 21613327.

View

Gpr126 is essential for peripheral nerve development and myelination in mammals.

Europe PMC ID: 21613327

View

Pohl et al. 2011. J Neurosci. 31(3):1069-80. PMID: 21248132.

View

Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.

Europe PMC ID: 21248132

View

Savvaki et al. 2010. J Neurosci. 30(42):13943-54. PMID: 20962216.

View

The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.

Europe PMC ID: 20962216

View

Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro.

Europe PMC ID: 20594620

View

Kawai et al. 2009. Eur J Neurosci. 30(11):2030-41. PMID: 20128842.

View

Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK.

Europe PMC ID: 20128842

View

Relucio et al. 2009. J Neurosci. 29(38):11794-806. PMID: 19776266.

View

Laminin alters fyn regulatory mechanisms and promotes oligodendrocyte development.

Europe PMC ID: 19776266

View

Homchaudhuri et al. 2009. Biochemistry. 48(11):2385-93. PMID: 19178193.

View

Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro.

Europe PMC ID: 19178193

View

Matsuo et al. 1997. Am J Pathol. 150(4):1253-66. PMID: 9094982.

View

Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.

Europe PMC ID: 9094982

View

Groome et al. 1988. J Neuroimmunol. 19(4):305-15. PMID: 2459156.

View

New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.

Europe PMC ID: 2459156

View

Hruby et al. 1987. Mol Immunol. 24(12):1359-64. PMID: 2448611.

View

Monoclonal antibodies reactive with myelin basic protein.

Europe PMC ID: 2448611

View

Groome et al. 1986. J Neuroimmunol. 12(4):253-64. PMID: 2428830.

View

Region-specific immunoassays for human myelin basic protein.

Europe PMC ID: 2428830

View

Elfman et al. 1986. J Neurochem. 46(2):509-15. PMID: 2416877.

View

Rat and mouse monoclonal antibodies to human myelin basic protein.

Europe PMC ID: 2416877

View

Add a reference

References: 49 entries

Grigoletto et al. 2017. Acta Neuropathol Commun. 5(1):37. PMID: 28482862.

View

Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss.

View

Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.

View

Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.

View

Fernandes et al. 2016. J Control Release. 238:300-310. PMID: 27369863.

View

Isoda et al. 2016. Neurosci Res. 110:18-28. PMID: 27083781.

View

Lim et al. 2016. Antioxidants (Basel). 5(3):. PMID: 27618111.

View

Protandim Protects Oligodendrocytes against an Oxidative Insult.

View

Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.

View

Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology.

View

Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions.

View

Crawford et al. 2016. Am J Pathol. 186(3):511-6. PMID: 26773350.

View

Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination.

View

Natrajan et al. 2015. Brain. 138(Pt 12):3581-97. PMID: 26463675.

View

Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.

View

HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.

View

SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.

View

Meade et al. 2015. Brain Res. 1611:101-13. PMID: 25842371.

View

Quantitative proteomic analysis of the brainstem following lethal sarin exposure.

View

Brügger et al. 2015. PLoS Biol. 13(9):e1002258. PMID: 26406915.

View

Müller et al. 2015. PLoS One. 10(8):e0136900. PMID: 26317513.

View

Pusic et al. 2014. J Neuroimmunol. 266(1-2):12-23. PMID: 24275061.

View

IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.

View

Horiuchi et al. 2012. Neurobiol Aging. 33(3):499-509. PMID: 20594620.

View

Time-lapse imaging of the dynamics of CNS glial-axonal interactions in vitro and ex vivo.

View

Ioannidou et al. 2012. PLoS One. 7(1):e30775. PMID: 22303455.

View

Monk et al. 2011. Development. 138(13):2673-80. PMID: 21613327.

View

Gpr126 is essential for peripheral nerve development and myelination in mammals.

View

Pohl et al. 2011. J Neurosci. 31(3):1069-80. PMID: 21248132.

View

Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.

View

Savvaki et al. 2010. J Neurosci. 30(42):13943-54. PMID: 20962216.

View

The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.

View

Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro.

View

Kawai et al. 2009. Eur J Neurosci. 30(11):2030-41. PMID: 20128842.

View

Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK.

View

Relucio et al. 2009. J Neurosci. 29(38):11794-806. PMID: 19776266.

View

Laminin alters fyn regulatory mechanisms and promotes oligodendrocyte development.

View

Homchaudhuri et al. 2009. Biochemistry. 48(11):2385-93. PMID: 19178193.

View

Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro.

View

Matsuo et al. 1997. Am J Pathol. 150(4):1253-66. PMID: 9094982.

View

Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.

View

Groome et al. 1988. J Neuroimmunol. 19(4):305-15. PMID: 2459156.

View

New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.

View

Hruby et al. 1987. Mol Immunol. 24(12):1359-64. PMID: 2448611.

View

Monoclonal antibodies reactive with myelin basic protein.

View

Groome et al. 1986. J Neuroimmunol. 12(4):253-64. PMID: 2428830.

View

Region-specific immunoassays for human myelin basic protein.

View

Elfman et al. 1986. J Neurochem. 46(2):509-15. PMID: 2416877.

View

Rat and mouse monoclonal antibodies to human myelin basic protein.

View

Add a reference

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Anti-Myelin Basic Protein [MBP40]

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References

References: 49 entries

References: 49 entries

Inventor Information

Invented at

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