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Anti-Myelin Basic Protein [MBP40]
Invented at Other
Anti-Myelin Basic Protein (region 82-87) [MBP12]
Invented at
- Datasheet
- References (51)
- Inventor Info
Info
| Catalogue Number | 153641 |
| Applications | ELISA FACS IHC IF WB |
| Antigen/Gene or Protein Targets | Myelin Basic Protein (region 82-87) |
| Synonyms |
Myelin basic protein, MBP, 20 kDa microtubule-stabilizing protein, Myelin A1 protein |
| Reactivity | Chicken, Human, Guinea Pig, Pig, Rat, Rabbit, Sheep and Mammals |
| Relevance | Myelin Basic Protein (MBP) is involved in the process of myelination of nerves in the nervous system. MBP Clone 12 recognizes an epitope in the 82-87 region of MBP, useful in clinical diagnosis to detect MBP levels or myelination in human MBP. MBP 12 is also used in a two-site ELISA with clone MBP 40. Both are used interchangeably as capture and detection antibody. |
| Host | Rat |
| Immunogen | Reacts with human MBP, recognizing epitopes in the 82-87 amino acid region (DENPVV) |
| Positive Control | Brain tissue |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 13-21 |
| Myeloma Used | NS0 |
| Notes |
Suggested dilutions for use Western Blot 1:100-1:2,000 Immunohistochemistry 1:10-1:500 ELISA 1:100-1:2,000 Immunofluorescence 1:10-1:500 Immunocytochemistry 1:5-1:1000 Research Areas: Neurobiology; Cell Type or Organelle Marker; Immunology; Genetics; Pathology; Stem Cell Biology |
| Research Area | Cell Type or Organelle Marker, Immunology, Neurobiology, Stem Cell Biology |
References: 51 entries
Grigoletto et al. 2017. Acta Neuropathol Commun. 5(1):37. PMID: 28482862.
Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss.
Europe PMC ID: 28482862
Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.
Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.
Fernandes et al. 2016. J Control Release. 238:300-310. PMID: 27369863.
Isoda et al. 2016. Neurosci Res. 110:18-28. PMID: 27083781.
Lim et al. 2016. Antioxidants (Basel). 5(3):. PMID: 27618111.
Protandim Protects Oligodendrocytes against an Oxidative Insult.
Europe PMC ID: 27618111
Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.
Europe PMC ID: 27417499
Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology.
Europe PMC ID: 27369863
Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions.
Europe PMC ID: 27083781
Crawford et al. 2016. Am J Pathol. 186(3):511-6. PMID: 26773350.
Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination.
Europe PMC ID: 26773350
Natrajan et al. 2015. Brain. 138(Pt 12):3581-97. PMID: 26463675.
Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.
Europe PMC ID: 26463675
HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.
Europe PMC ID: 26406915
SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.
Europe PMC ID: 26317513
Meade et al. 2015. Brain Res. 1611:101-13. PMID: 25842371.
Quantitative proteomic analysis of the brainstem following lethal sarin exposure.
Europe PMC ID: 25842371
Brügger et al. 2015. PLoS Biol. 13(9):e1002258. PMID: 26406915.
Müller et al. 2015. PLoS One. 10(8):e0136900. PMID: 26317513.
Pusic et al. 2014. J Neuroimmunol. 266(1-2):12-23. PMID: 24275061.
IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.
Europe PMC ID: 24275061
Horiuchi et al. 2012. Neurobiol Aging. 33(3):499-509. PMID: 20594620.
Time-lapse imaging of the dynamics of CNS glial-axonal interactions in vitro and ex vivo.
Europe PMC ID: 22303455
Ioannidou et al. 2012. PLoS One. 7(1):e30775. PMID: 22303455.
Monk et al. 2011. Development. 138(13):2673-80. PMID: 21613327.
Gpr126 is essential for peripheral nerve development and myelination in mammals.
Europe PMC ID: 21613327
Pohl et al. 2011. J Neurosci. 31(3):1069-80. PMID: 21248132.
Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.
Europe PMC ID: 21248132
Savvaki et al. 2010. J Neurosci. 30(42):13943-54. PMID: 20962216.
The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.
Europe PMC ID: 20962216
Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro.
Europe PMC ID: 20594620
Kawai et al. 2009. Eur J Neurosci. 30(11):2030-41. PMID: 20128842.
Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK.
Europe PMC ID: 20128842
Relucio et al. 2009. J Neurosci. 29(38):11794-806. PMID: 19776266.
Laminin alters fyn regulatory mechanisms and promotes oligodendrocyte development.
Europe PMC ID: 19776266
Homchaudhuri et al. 2009. Biochemistry. 48(11):2385-93. PMID: 19178193.
Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro.
Europe PMC ID: 19178193
Van der Goes et al. 1999. J Neuroimmunol. 101(1):61-7. PMID: 10580814.
FACS
The role of anti-myelin (auto)-antibodies in the phagocytosis of myelin by macrophages.
Europe PMC ID: 10580814
Matsuo et al. 1997. Am J Pathol. 150(4):1253-66. PMID: 9094982.
Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.
Europe PMC ID: 9094982
Groome et al. 1988. J Neuroimmunol. 19(4):305-15. PMID: 2459156.
New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
Europe PMC ID: 2459156
Hruby et al. 1987. Mol Immunol. 24(12):1359-64. PMID: 2448611.
Monoclonal antibodies reactive with myelin basic protein.
Europe PMC ID: 2448611
Groome et al. 1986. J Neuroimmunol. 12(4):253-64. PMID: 2428830.
Region-specific immunoassays for human myelin basic protein.
Europe PMC ID: 2428830
Elfman et al. 1986. J Neurochem. 46(2):509-15. PMID: 2416877.
Rat and mouse monoclonal antibodies to human myelin basic protein.
Europe PMC ID: 2416877
Add a reference
References: 51 entries
Grigoletto et al. 2017. Acta Neuropathol Commun. 5(1):37. PMID: 28482862.
Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss.
Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.
Friess et al. 2016. Cell Calcium. 60(5):322-330. PMID: 27417499.
Fernandes et al. 2016. J Control Release. 238:300-310. PMID: 27369863.
Isoda et al. 2016. Neurosci Res. 110:18-28. PMID: 27083781.
Lim et al. 2016. Antioxidants (Basel). 5(3):. PMID: 27618111.
Protandim Protects Oligodendrocytes against an Oxidative Insult.
Intracellular ion signaling influences myelin basic protein synthesis in oligodendrocyte precursor cells.
Part II: Functional delivery of a neurotherapeutic gene to neural stem cells using minicircle DNA and nanoparticles: Translational advantages for regenerative neurology.
Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions.
Crawford et al. 2016. Am J Pathol. 186(3):511-6. PMID: 26773350.
Pre-Existing Mature Oligodendrocytes Do Not Contribute to Remyelination following Toxin-Induced Spinal Cord Demyelination.
Natrajan et al. 2015. Brain. 138(Pt 12):3581-97. PMID: 26463675.
Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.
HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.
SncRNA715 Inhibits Schwann Cell Myelin Basic Protein Synthesis.
Meade et al. 2015. Brain Res. 1611:101-13. PMID: 25842371.
Quantitative proteomic analysis of the brainstem following lethal sarin exposure.
Brügger et al. 2015. PLoS Biol. 13(9):e1002258. PMID: 26406915.
Müller et al. 2015. PLoS One. 10(8):e0136900. PMID: 26317513.
Pusic et al. 2014. J Neuroimmunol. 266(1-2):12-23. PMID: 24275061.
IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.
Horiuchi et al. 2012. Neurobiol Aging. 33(3):499-509. PMID: 20594620.
Time-lapse imaging of the dynamics of CNS glial-axonal interactions in vitro and ex vivo.
Ioannidou et al. 2012. PLoS One. 7(1):e30775. PMID: 22303455.
Monk et al. 2011. Development. 138(13):2673-80. PMID: 21613327.
Gpr126 is essential for peripheral nerve development and myelination in mammals.
Pohl et al. 2011. J Neurosci. 31(3):1069-80. PMID: 21248132.
Genetically induced adult oligodendrocyte cell death is associated with poor myelin clearance, reduced remyelination, and axonal damage.
Savvaki et al. 2010. J Neurosci. 30(42):13943-54. PMID: 20962216.
The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.
Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro.
Kawai et al. 2009. Eur J Neurosci. 30(11):2030-41. PMID: 20128842.
Maintenance of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK.
Relucio et al. 2009. J Neurosci. 29(38):11794-806. PMID: 19776266.
Laminin alters fyn regulatory mechanisms and promotes oligodendrocyte development.
Homchaudhuri et al. 2009. Biochemistry. 48(11):2385-93. PMID: 19178193.
Influence of membrane surface charge and post-translational modifications to myelin basic protein on its ability to tether the Fyn-SH3 domain to a membrane in vitro.
Van der Goes et al. 1999. J Neuroimmunol. 101(1):61-7. PMID: 10580814.
FACS
The role of anti-myelin (auto)-antibodies in the phagocytosis of myelin by macrophages.
Matsuo et al. 1997. Am J Pathol. 150(4):1253-66. PMID: 9094982.
Unmasking of an unusual myelin basic protein epitope during the process of myelin degeneration in humans: a potential mechanism for the generation of autoantigens.
Groome et al. 1988. J Neuroimmunol. 19(4):305-15. PMID: 2459156.
New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
Hruby et al. 1987. Mol Immunol. 24(12):1359-64. PMID: 2448611.
Monoclonal antibodies reactive with myelin basic protein.
Groome et al. 1986. J Neuroimmunol. 12(4):253-64. PMID: 2428830.
Region-specific immunoassays for human myelin basic protein.
Elfman et al. 1986. J Neurochem. 46(2):509-15. PMID: 2416877.
Rat and mouse monoclonal antibodies to human myelin basic protein.
Add a reference