- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||Msh3|
|Parental Line||SVG-A immortalized human astrocytes|
|Synonyms||DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, DUP, Mismatch repair protein 1, MRP1|
|Disease Keywords||Neurodegenerative disease such as Huntington’s|
The MSH3 gene encodes a DNA mismatch repair protein important in certain cancer types and in certain neurodegenerative diseases.
Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.
While ~98% of Msh3 protein is lost in these cells, the abundance of the related proteins Msh2 and Msh6 appear unaffected. This is relevant to DNA mismatch repair.
|Production Details||Exon 2 of the MSH3 gene was targeted by CRISPR/Cas9. The resulting Msh3-/- cell line encodes a 3 amino acid deletion in Msh3 that results in 98% loss of Msh3 protein, as judged by western blots with two different antibodies.|
|Research Area||Cancer, Cell Cycle, DNA Damage and Repair|
|Growth/Phenotype Keywords||Adherent cell line|
|Recommended Growing Conditions||DMEM supplemented with 10% FBS|
|Positive Control||SVG-A Cell Line, SVG-A Msh3 1.7X Cell Line (derived from Msh3-/- SVG-A Cell Line)|
DNA repair assays
Trinucleotide expansion assay
Cell background for rescue with wild type or variant Msh3 clones
Immunoprecipitation control for Msh2-Msh3 protein complex
Functional analysis of Msh3 variants
CRISPR edited cells.
Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.
This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.
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