- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||Sufu (UniProt ID: Q9UMX1)|
|Synonyms||Suppressor of fused homolog, SUFUH|
|Reactivity||Human and Mouse|
Anti-phospho-Sufu polyclonal antibody recognises wild type sufu which is phosphorylated at serine 352. The antibody specificity for the phosphorylated version has been confirmed; it does not react against a non-phosphorylated version of Sufu which has serine 352 substituted by alanine.
Sufu is a negative regulator in the hedgehog signaling pathway which directs cell proliferation and patterning during embryogenesis. Sufu down-regulates GLI1- and GLI2-mediated transactivation of target genes and is part of a corepressor complex that acts on DNA-bound GLI1. Required for normal embryonic development. Sufu is ubiquitous in adult tissues.
Heterozygous loss of Sufu, in conjunction with the loss of p53, leads to the development of medulloblastoma and rhabdomyosarcoma. Somatic Sufu mutations have been identified in multiple other malignancies, including prostate cancer.
|Immunogen||Sufu peptide: Hu #347~360: CLESDS-pS-pT-AIIPHEL|
|Method of Purification||This rabbit polyclonal antibody was raised against the following peptide of Sufu: Hu #347~360: CLESDS-pS-pT-AIIPHEL. To ensure the specific recognition of phosphorylated Sufu, the antibody was affinity-purified against a phosphorylated peptide and absorbed against a non-phosphorylated peptide CLESDSSTAIIPHEL.|
|Molecular Weight (kDa)||~65|
Western Blot: 1:500
|Research Area||Cancer, Developmental Biology, Gene Expression|
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