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NUOC-1 Cell Line

Info

Catalogue Number 153527
Antigen/Gene or Protein Targets Ovarian cancer
Parental Line Ascites of chemotherapy naive patient
Synonyms HGSC, high grade mixed ovarian cancer, epithelial ovarian cancer
Host Human
Tissue Ovary
Disease Keywords High Grade Ovarian Cancer
Model Cancer Model
Relevance Epithelial ovarian cancer is diagnosed at late disease stage in more than 70% of patients. Ovarian cancer is the leading cause of gynaecological cancer mortality worldwide with a 5-year overall survival of 30–39%. It has long been recognised by clinicians that ovarian cancer is a set of heterogeneous diseases. NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy naïve patient with a primary mixed endometrioid/ clear cell/ high grade serous ovarian cancer
Production Details Ascites were collected from patients undergoing surgery for ovarian cancer. 20 ml of ascites was added to 20 ml of warmed culture medium in T75 flask, and incubated at 37°C, 5% CO2, 95% humidified air. The medium was aspirated and 13 ml of warmed fresh medium was replaced on day 3 to 5. The medium was replaced every 4 to 5 days until the cells approached confluence
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Doubling time approximately 58 hours
Recommended Growing Conditions RPMI 1640 medium supplemented with 20% FCS, 20 mM L-glutamine and 1% penicillin and streptomycin
Notes NUOC-1 cells grow as an adherent monolayer.
The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression.
NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective.
Karyotype analysis demonstrated a complex tetraploid karyotype

References

There are 2 reference entries for this reagent.

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References: 2 entries

McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.

Functional characterisation of a novel ovarian cancer cell line, NUOC-1.

Europe PMC ID: 28460465


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References: 2 entries

McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.

Functional characterisation of a novel ovarian cancer cell line, NUOC-1.


Add a reference

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