NUOC-1 Cell Line
Invented at Northern Institute For Cancer Research, Newcastle University
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 153527 |
| Antigen/Gene or Protein Targets | Ovarian cancer |
| Parental Line | Ascites of chemotherapy naive patient |
| Synonyms | HGSC, high grade mixed ovarian cancer, epithelial ovarian cancer |
| Host | Human |
| Tissue | Ovary |
| Disease Keywords | High Grade Ovarian Cancer |
| Model | Cancer Model |
| Relevance | Epithelial ovarian cancer is diagnosed at late disease stage in more than 70% of patients. Ovarian cancer is the leading cause of gynaecological cancer mortality worldwide with a 5-year overall survival of 30–39%. It has long been recognised by clinicians that ovarian cancer is a set of heterogeneous diseases. NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy naïve patient with a primary mixed endometrioid/ clear cell/ high grade serous ovarian cancer |
| Production Details | Ascites were collected from patients undergoing surgery for ovarian cancer. 20 ml of ascites was added to 20 ml of warmed culture medium in T75 flask, and incubated at 37°C, 5% CO2, 95% humidified air. The medium was aspirated and 13 ml of warmed fresh medium was replaced on day 3 to 5. The medium was replaced every 4 to 5 days until the cells approached confluence |
| Conditional | No |
| Research Area | Cancer, Drug Discovery & Development |
| Growth/Phenotype Keywords | Doubling time approximately 58 hours |
| Recommended Growing Conditions | RPMI 1640 medium supplemented with 20% FCS, 20 mM L-glutamine and 1% penicillin and streptomycin |
| Notes |
NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype |
References: 2 entries
McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.
Functional characterisation of a novel ovarian cancer cell line, NUOC-1.
Europe PMC ID: 28460465
Add a reference
References: 2 entries
McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.
Functional characterisation of a novel ovarian cancer cell line, NUOC-1.
Add a reference
